Inhibitory Effect of Wharton Jelly Derived Mesenchymal Stem Cells on Chronic Myeloid Cancer Cell Lines

Abstract

Mesenchymal stem cells (MSCs) have the capacity for self‐renewal and pluripotency, making them a primary candidate for cell‐based therapy. The purpose of this study was toevaluate inhibition effect of Wharton Jelly derived MSC (WJ‐MSC) on the K562 cells as chronic myeloid leukemia (CML) cell lines. In this study, WJ‐MSCs were isolated from an umbilicalcord, and characterized by flow cytometry. WJ‐MSCs (1×104/cm2) were obtained from Passage 1, and plated into 24‐well plates in 1 ml DMEM complete culture medium solution.WJ‐MSCs were incubated in a flask at 37˚C in a humidified air with 5% CO2. After 7 hours, K562 cells (1×105/ml) were added respectively K562: MSC; 1:2, 1:5, 1:10 ratio, low‐glucoseDMEM containing 10% FBS and 1% penicillin/streptomycin solution. The co‐culture was incubated 72 hours at 37˚C in 5% CO2, and the co‐cultured K562 cells were subsequentlyseparated from adherent WJ‐MSCs by slow and careful pipetting. The cell counts and viability were determined by trypan blue dye exclusion. The viable and non‐viable cells werecounted with a hemocytometer. The non‐viable cell percentages were compared as 1:2 (42%), 1:5 (52,2%), 1:10 (33,3%) and control plates (%7,69) (untreated K562 cell line) after 72hours incubation. The most effective dose was found 1:5 (52,2%). In conclusion, the results showed that WJ‐MSCs can suppress K562 cell proliferation when using in proper ratio. Thisstudy will provide perspective on the next projects. In the future, WJ‐MSCs may be an option for their clinic use for the inhibition of cancer cells.Keywords: Cancer immunology, cellular interactions, stem cells