PCR Assay for Identification of Animal Species in Different Ready to Eat Raw Meat Samples

Abstract

In this study, 500 ready to eat raw meat samples (minced meat, lahmacun ingredients, kebap, stew and meatball samples) analyzed for different animal originated DNA residues (pork, chicken, cattle, sheep, horse, donkey, cat, dog, mouse, cockroach and house fly) by PCR procedures. Besides, all the samples were analyzed for important foodborne pathogens (coliforms, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Salmonella spp.). According to the results, total of 52 samples were determined as adulterated and different originated animal DNA samples were found (chicken, horse and sheep DNA residues). Adulterated samples were also determined more risky for the consumers in microbiological aspect.