NEXT GENERATION SEQUENCING TECHNOLOGIES AND USAGE IN FORENSIC GENETICS

Abstract

DNA sequence analysis applications, which started, all the way back in the 90s and have stepped up with the Human Genome Project, has reached a completely different level with the techniques and methods developed nowadays. Almost all fields of application have taken advantage of these developments where single base level and genome distributed DNA sequence differences can be detected in maternal twins [1]. Forensic Genetics did not keep distance from these developments that started with clinical purpose and with the New Generation Sequencing techniques developed in recent years, it is stated that a new era in this field has begun. Particularly the convenience provided by the analysis of mix samples containing DNA of multiple persons and due to presenting more than one genetic markers in a single analysis, these technologies have brought great dynamism to the field. Once the foremost disadvantages of New Generation Sequencing methods situated as the cost that comes with high technology and analysis errors of high amounts of information produced are overcome, a paradigm shift is inevitable in where "length polymorphism" dominates the field of forensic genetics [2].