Relationship between CD107a expression and cytotoxic activity

Abstract

NK cells play important roles in innate immunity against tumors and infections of the host. Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8(+) T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56(+) NK, CD8(+) T cells and lymphocytes has been determined after various stimuli. Effector cells from PBMCs of healthy subjects were isolated and K562 cell line was used as target of cytotoxicity. IL-2 stimulation resulted in a significant increase of CD107a expression in CID56(+) NK, CD8(+) T cells and lymphocytes. Increased expression of CD107a after IL-2 stimulation of NK cells was parallel to the increase of cytotoxicity. Our results suggest that CD107a expression may be a sensitive marker for the cytotoxic activity determination. (c) 2008 Elsevier Inc. All rights reserved.