Prostaglandin-E1 has a protective effect on renal ischemia/reperfusion-induced oxidative stress and inflammation mediated gastric damage in rats.

Abstract

Gastrointestinal complications are frequent in renal transplant recipients. In this regard, renal ischemia/reperfusion injury (IRI)-induced gastric damage seems to be important and there is no data available on the mechanism of this pathology. Because of its anti-inflammatory and anti-oxidant properties, it can be suggested that prostaglandin-E-1 (PGE(1)) protects cells from renal IRI-induced gastric damage. The aim of this study was to investigate the molecular mechanisms of gastric damage induced by renal IRI and the effect of PGE(1) on these mechanisms. We set an experiment with four different animal groups: physiological saline-injected and sham-operated rats, PGE(1) (20 mu g/kg)-administered and sham operated rats, renal IRI subjected rats, and PGE(1)-radministered and renal IRI subjected rats. The protective effect of PGE(1) on renal IRI-induced gastric damage was determined based on reduced histological damage and lactate dehydrogenase activity. Moreover, we demonstrated that PGE(1) shows its protective effect through reducing the production of reactive oxygen species and malondialdehyde levels. During histological examination, we observed the presence of common mononuclear cell infiltration. Therefore, pro-inflammatory cytokines tumor necrosis factor-alpha and interleukin-1 beta levels were measured and it has been shown that PGE(1) suppressed both cytokines. Furthermore, it was found that PGE(1) reduced the number of NF-kappa B+ and caspase-3(+) inflammatory cells, and also NF-kappa B DNA-binding activity, while increasing proliferating cell nuclear antigen epithelial cells in the stomach tissue of rats subjected to renal IR. Our data showed that PGE(1), has a protective effect on renal IRI-induced oxidative stress and inflammation mediated gastric damage in rats. (C) 2016 Elsevier B.V. All rights reserved.