IDENTIFICATION OF VOLATILE COMPOUNDS AND ANTIMICROBIAL ACTIVITIES OF MUSCARI NEGLECTUM GROWING IN TURKEY

Abstract

The absolute essential oils obtained from the bulbs and the aerial parts of M. neglectum collected from Kahramanmaras (Turkey) were analysed by GC-FID and GC-MS. In GC-MS analyses, 17 components comprising 100% of the essential oil of the bulbs and 17 components comprising 97.1% of the essential oil of the aerial parts. Major constituents of the absolute essential oil of the bulbs were found to be ethyl palmitate (18.7%), ethyl linoleate (16.3%), palmitic acid (15.2%) and linoleic acid (10.8%). The aerial parts oil mainly contents palmitic acid (28.5%), oleic acid (10.9%), linoleic acid (22.6%) and linolenic acid (11.4%). The absolute essential oils were analyzed by using microdilution assay for antimicrobial activity against three Gram-positive, four Gram-negative bacteria and one of fungus. The oils obtained from bulbs and aerial parts possessed activity against Staphylococcus epidermidis with MIC values of 625 and 1250 mu g/mL, respectively. The bulbs oil showed antibacterial activity against Enterococcus faecalis and Escherichia coli with 156.2 mu g/mL and 625 mu g/mL MIC values. The bulbs oil exhibited antifungal activity against Candida albicans with 312.5 mu g/mL MIC value. This is the first report on the chemical composition and antimicrobial activity of the essential oils obtained from M. neglectum growing in Turkey.