Novel Optical Fiber Reflectometric CUPRAC Sensor for Total Antioxidant Capacity Measurement of Food Extracts and Biological Samples

Abstract

A novel fiber optic sensor was developed for screening the total antioxidant capacity (TAC) based on the use of cupricneocuproine (Cu(II)Nc) immobilized onto a Nafion cation-exchange membrane with reflectance spectrometric measurement. The reflectance change associated with the formation of the highly colored Cu(I)Nc chelate on the membrane as a result of reaction with antioxidants was measured at 530 nm by using a miniature reflectance spectrometer. The calibration graph of trolox (TR) was linear with a slope of 3.40 x 10(3) L mol(-1) mm(-1). The limit of detection (LOD) and limit of quantification (LOQ) for TR in the reflectometric cupric reducing antioxidant capacity (CUPRAC) method were found as 0.53 and 1.76 mu M, respectively. The trolox equivalent antioxidant capacities (TEAC) of various antioxidant compounds using the proposed method were comparable to those of the main CUPRAC assay. This assay was validated through linearity, additivity, precision, and recovery. The developed reflectance sensor was used to screen the TAC of some commercial fruit juices and mice tissue homogenates without preliminary treatment. The method is rapid, inexpensive, versatile, and nonlaborious, uses stable reagents on the sensor, and enables the in situ estimation of antioxidant capacity of food extracts and biological samples.