Evaluation of Melatonin and Vitamin D’s Effects on Zoledronic Acid Treated Osteoblasts’ Vitality, in Vitro

Abstract

Objective: In this research, it was aimed to evaluate the effects ofmelatonin and vitamin D on zoledronic acid treated human osteoblasts’ vitality.Our main purpose in this study is try to find an answer to “Can supplement usagesuch as melatonin and vitamin D reduce the severity of MRONJ?”Methods: Human osteoblasts incubated various zoledronic acidconcentrations (10 and 50 μM). Two different concentrations of vitamin D (10and 100 μM) and melatonin (40 μM) were added in several combinations for 24thand 48th hour and 36 groups were created. After that cells are collected and theeffects are tested with the use of the proliferation assay (MTT). Data werecollected and analyzed statistically.Results: There were statistically significant differences between 24th and48th hour’s results.Conclusion: Generally, after 24th hour melatonin and vitamin D additionto zoledronic acid treated human osteoblast can contribute to reset negative effectsof zoledronic acid. Further investigations are needed to suggest melatonin andvitamin d supplements to MRONJ patients both in vivo and in vitro.