Flow Cytometric Crossmatching and Panel-Reactive Antibodies in Chronic Renal Failure Patients

Abstract

Aim. Anti-donor antibodies, denoted as "panel-reactive antibodies" (PRAs), are one of the most important factors influencing graft survival after renal transplantation. PRA is generally analyzed with enzyme-linked immunosorbent assay or flow cytometry (FC), which identify the HLA antigen specific for the preformed antibody.