Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF)
Date
2015Author
Ay, Nur Zeynep
Onder, Tamer T.
Ozlu, Can
Ruacan, Arzu
Gül, Ahmet
Fidan, Kerem
Kavaklioglu, Gulnihal
Ebrahimi, Ayyub
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Fibroblasts from a Familial Mediterranean Fever (FMF) patient were reprogrammed with episomal vectors by using the Neon Transfection System for the generation of integration-free induced pluripotent stem cells (iPSCs). The resulting iPSC line was characterized to determine the expression of pluripotency markers, proper differentiation into three germ layers, the presence of normal chromosomal structures as well as the lack of genomic integration. A homozygous missense mutation in the MEFV gene (p.Met694Val), which lead to typical FMF phenotype, was shown to be present in the generated iPSC line. (C) 2015 The Authors. Published by Elsevier B.V.
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