The effect of 24-epibrassinolide on the development of spinach (Spinacia oleracea L. var. matador) seedlings

Abstract

Apart from auxin, gibberellin, cytokine, absisic acid and ethylene, which are known to regulate plant growth and development, strong evidence has been obtained to add brassinosteroids to this group in recent years. Brassinosteroids affect different processes such as plant growth and development, seed germination, rhizogenesis, flowering, senescence, abscission and ripening. In this study, the effect of epibrassinolid, which is an active form of brassinosteroid, on growth and development of spinach seedlings was investigated. For this purpose, 15-day spinach seedlings (Spinacia oleracea L. cv. Matador) were taken into Hoagland solution and divided into 2 groups. First group as a control group (Hoagland) and second group as an experimental (treated) group (Hoagland +24 EBL) was called. 10-9 M 24-eBL was applied to the treated group seedlings until 45th days (for 30 days) by spraying method (all leaves except root). The control group was sprayed with distilled water only. In the following period, biochemical analyzes were performed on the leaves of the seedlings harvested on the 45th day. As a result of the analysis, the eBL applied experimental group compared to the untreated control group; fresh weight, total soluble protein and total chlorophyll content decreased in the treated seedlings. Peroxidase and superoxide dismutase enzyme activities increased, and membrane permeability changes were observed. This study was designed to investigate the effect of epibrassinolidine on the development of seedlings. Keywords: Spinach (Spinacia oleracea L. cv. Matador), Hoagland, 24-Epibrassinolide, Antioxidative enzyme.