THE ANTIBIOTIC RESISTANCE GENES IN ESCHERICHIA COLI ISOLATES FROM NARMAN LANDFILL (NL) AREA IN ERZURUM, TURKEY

Abstract

In this study, we aimed to investigate four antibiotic resistance genes (ARGs) [beta-lactams resistant genes (bla(SHV) and bla(CTX-m)), and tetracycline resistant genes (tetA and tetB)] in Escherichia coli isolates by PCR. For this purpose, samples were collected with refuse sampling technique (AK1, AK2, AK3, AK4) from Narman Landfill (NL, Erzurum, Narman) and from surface water of streams (D1, D2, D3, NI, N2) and around the natural environment of landfill (C1, K1), seasonally. Firstly, we used culture-dependent methods and molecular techniques to detect and quantify antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in Escherichia coli.