dc.contributor.author | Ulutin, T | |
dc.contributor.author | Guven, GS | |
dc.contributor.author | Guven, M | |
dc.contributor.author | Onaran, H | |
dc.contributor.author | Hacianefioglu, S | |
dc.date.accessioned | 2021-03-04T19:43:01Z | |
dc.date.available | 2021-03-04T19:43:01Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Guven G., Guven M., Onaran H., Ulutin T., Hacianefioglu S., "Individual sensitivity to cytogenetic effects of benzo[alpha]pyrene in cultured human lymphocytes: Influence of glutathione S-transferase M1 genotype", GENETICS AND MOLECULAR BIOLOGY, cilt.29, ss.142-147, 2006 | |
dc.identifier.issn | 1415-4757 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_911cf12c-d401-4b84-8e86-dd9889a2edde | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/97919 | |
dc.identifier.uri | https://doi.org/10.1590/s1415-47572006000100027 | |
dc.description.abstract | Sister chromatid exchange (SCE) and chromosome aberrations (CA) in peripheral lymphocytes has been widely used in assessing exposure to mutagens and carcinogens. One of the extensively studied genotoxins is benzo[a]pyrene (BaP). We studied the ability of BaP to induce SCE and CA in 16 glutathione S-transferase M1 (GSTM1)-positive and 15 GSTM1-null individuals by analyzing 72-h whole-blood lymphocyte cultures, either BaP-untreated (controls) or treated with 5 mu M of BaP for 24 or 48 h. There was no differences in the level of BaP-induced chromosomal aberrations between GSTM1-positive or null individuals when the cells were BaP-exposed for 24h (0.083 +/- 0.059 vs. 0.090 +/- 0.058) or 48h (0.092 +/- 0.057 vs. 0.096 +/- 0.050. The frequency of SCE in controls was GSTM1-positive = 2.96 +/- 0.35 and GSTM1-null = 3.23 +/- 0.56 while that for BaP-treated lymphocytes was GSTM1-positive = 5.56 +/- 0.83 and GSTM1-null = 6.09 +/- 1.11 and were not statistically significant. The rates of BaP-induced in vitro chromatid and chromosome-type gaps and breaks were similar in all groups, although GSTM1-null genotype chromatid-type breaks were more frequent (0.064 +/- 0.039 per metaphase) than chromosome-type breaks (0.032 +/- 0.027 per metaphase) after 48 h treatment with BaP (p < 0.001). These findings suggest that BaP-induced in vitro SCE and CA are not influenced by the GSTM1 genotype. | |
dc.language.iso | eng | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Sitogenetik | |
dc.subject | Temel Bilimler | |
dc.subject | Tıp | |
dc.subject | Tıbbi Genetik | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Dahili Tıp Bilimleri | |
dc.subject | GENETİK VE HAYAT | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.title | Individual sensitivity to cytogenetic effects of benzo[alpha]pyrene in cultured human lymphocytes: Influence of glutathione S-transferase M1 genotype | |
dc.type | Makale | |
dc.relation.journal | GENETICS AND MOLECULAR BIOLOGY | |
dc.contributor.department | , , | |
dc.identifier.volume | 29 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 142 | |
dc.identifier.endpage | 147 | |
dc.contributor.firstauthorID | 178160 | |