Oxidative stress and cannabinoid receptor expression in type-2 diabetic rat pancreas following treatment with Delta(9)-THC

Abstract

The objectives of study were (a) to determine alteration of feeding, glucose level and oxidative stress and (b) to investigate expression and localization of cannabinoid receptors in type-2 diabetic rat pancreas treated with (9)-tetrahydrocannabinol ((9)-THC). Rats were randomly divided into four groups: control, (9)-THC, diabetes and diabetes+(9)-THC groups. Diabetic rats were treated with a single dose of nicotinamide (85mg/kg) 15min before injection of streptozotocin (65mg/kg). (9)-THC was administered intraperitoneally at 3mg/kg/day for 7days. Body weights and blood glucose level of rats in all groups were measured on days0, 7, 14 and 21. On day15 after the (9)-THC injections, pancreatic tissues were removed. Blood glucose levels and body weights of diabetic rats treated with (9)-THC did not show statistically significant changes when compared with the diabetic animals on days7, 14 and 21. Treatment with (9)-THC significantly increased pancreas glutathione levels, enzyme activities of superoxide dismutase and catalase in diabetes compared with non-treatment diabetes group. The cannabinoid 1 receptor was found in islets, whereas the cannabinoid 2 receptor was found in pancreatic ducts. Their localization in cells was both nuclear and cytoplasmic. We can suggest that (9)-THC may be an important agent for the treatment of oxidative damages induced by diabetes. However, it must be supported with anti-hyperglycaemic agents. Furthermore, the present study for the first time emphasizes that (9)-THC may improve pancreatic cells via cannabinoid receptors in diabetes. The aim of present study was to elucidate the effects of (9)-THC, a natural cannabinoid receptor agonist, on the expression and localization of cannabinoid receptors, and oxidative stress statue in type-2 diabetic rat pancreas. Results demonstrate that the cannabinoid receptors are presented in both Langerhans islets and duct regions. The curative effects of (9)-THC can be occurred via activation of cannabinoid receptors in diabetic rat pancreas. Moreover, it may provide a protective effect against oxidative damage induced by diabetes. Thus, it is suggested that (9)-THC can be a candidate for therapeutic alternatives of diabetes symptoms. Copyright (c) 2014 John Wiley & Sons, Ltd.