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dc.contributor.authorDalay, N
dc.contributor.authorErten, N
dc.contributor.authorAkisik, Ebru Esin
dc.contributor.authorDeligezer, Uğur
dc.date.accessioned2021-03-04T11:21:19Z
dc.date.available2021-03-04T11:21:19Z
dc.date.issued2006
dc.identifier.citationDeligezer U., Erten N., Akisik E. E. , Dalay N., "Circulating fragmented nucleosomal DNA and caspase-3 mRNA in patients with lymphoma and myeloma", EXPERIMENTAL AND MOLECULAR PATHOLOGY, cilt.80, sa.1, ss.72-76, 2006
dc.identifier.issn0014-4800
dc.identifier.othervv_1032021
dc.identifier.otherav_71e86253-66e3-44ea-9efc-a941ae13eea9
dc.identifier.urihttp://hdl.handle.net/20.500.12627/78413
dc.identifier.urihttps://doi.org/10.1016/j.yexmp.2005.05.001
dc.description.abstractElevated amounts of cell-free nucleic acids are detected in the circulation of cancer patients. The type and pattern of these may vary depending on the origin. Recently, we described the presence of circulating fragmented nucleosomal DNA. In the present study, our aim was to investigate the association between nucleosomal DNA, caspase-3 expression and circulating caspase-3 mRNA, the primary activator of nucleosomal DNA fragmentation. DNA fragmentation was analyzed by gel electrophoresis, and caspase-3 was analyzed by RT-PCR. Plasma samples from 65% of patients were positive for nucleosomal DNA fragmentation, whereas none of the healthy controls showed DNA fragmentation (P = 0.0004). Expression of caspase-3 differed significantly between the cases and controls (P < 0.0001). However, we observed no direct correlation between nucleosomal DNA fragmentation and caspase-3 expression in lymphocytes (P = 0.145). Circulating plasma caspase-3 mRNA was detected by nested RT-PCR, and no significant difference was observed between the patients and the control group (P = 0.5). Our results indicate that caspase-3 expression is increased in lymphocytes from patients. When compared to healthy individuals, no differences were observed in the amount of circulating mRNA. These findings suggest that nucleosomal DNA fragmentation is not correlated with elevated levels of apoptosis and circulating caspase-3 mRNA in circulating tumor cells. (c) 2005 Elsevier Inc. All rights reserved.
dc.language.isoeng
dc.subjectPatoloji
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectCerrahi Tıp Bilimleri
dc.subjectTıp
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectBiyoloji ve Biyokimya
dc.subjectPATOLOJİ
dc.titleCirculating fragmented nucleosomal DNA and caspase-3 mRNA in patients with lymphoma and myeloma
dc.typeMakale
dc.relation.journalEXPERIMENTAL AND MOLECULAR PATHOLOGY
dc.contributor.department, ,
dc.identifier.volume80
dc.identifier.issue1
dc.identifier.startpage72
dc.identifier.endpage76
dc.contributor.firstauthorID177721


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