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dc.contributor.authorZeidan-Chulia, Fares
dc.contributor.authorSyrjanen, Stina
dc.contributor.authorGursoy, Mervi
dc.contributor.authorGursoy, Ulvi K.
dc.contributor.authorFiratli, Erhan
dc.contributor.authorKasnak, Goekhan
dc.contributor.authorKononen, Eija
dc.date.accessioned2021-03-04T08:11:39Z
dc.date.available2021-03-04T08:11:39Z
dc.identifier.citationKasnak G., Kononen E., Syrjanen S., Gursoy M., Zeidan-Chulia F., Firatli E., Gursoy U. K. , "NFE2L2/NRF2, OGG1, and cytokine responses of human gingival keratinocytes against oxidative insults of various origin", MOLECULAR AND CELLULAR BIOCHEMISTRY, cilt.452, ss.63-70, 2019
dc.identifier.issn0300-8177
dc.identifier.othervv_1032021
dc.identifier.otherav_62136eeb-9bf7-424e-8068-ecca4d093c38
dc.identifier.urihttp://hdl.handle.net/20.500.12627/68317
dc.identifier.urihttps://doi.org/10.1007/s11010-018-3412-y
dc.description.abstractObjectiveBacterial or tobacco-related insults induce oxidative stress in gingival keratinocytes. The aim of this study was to investigate anti-oxidative and cytokine responses of human gingival keratinocytes (HMK cells) against Porphyromonas gingivalis lipopolysaccharide (Pg LPS), nicotine, and 4-nitroquinoline N-oxide (4-NQO).Materials and methodsHMK cells were incubated with Pg LPS (1 mu l/ml), nicotine (1.54mM), and 4-NQO (1 mu M) for 24h. Intracellular and extracellular levels of interleukin (IL)-1, IL-1 receptor antagonist (IL-1Ra), IL-8, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF) were measured with the Luminex (R) xMAP technique, and nuclear factor, erythroid 2 like 2 (NFE2L2/NRF2) and 8-oxoguanine DNA glycosylase (OGG1) with Western blots. Data were statistically analyzed by two-way ANOVA with Bonferroni correction.ResultsAll tested oxidative stress inducers increased intracellular OGG1 levels, whereas only nicotine and 4-NQO induced NFE2L2/NRF2 levels. Nicotine, 4-NQO, and their combinational applications with Pg LPS induced the secretions of IL-1 and IL-1Ra, while that of IL-8 was inhibited by the presence of Pg LPS. MCP-1 secretion was suppressed by nicotine, alone and together with Pg LPS, while 4-NQO activated its secretion. Treatment of HMK cells with Pg LPS, nicotine, 4-NQO, or their combinations did not affect VEGF levels.ConclusionPg LPS, nicotine, and 4-NQO induce oxidative stress and regulate anti-oxidative response and cytokine expressions in human gingival keratinocytes differently. These results may indicate that bacterial and tobacco-related insults regulate distinct pathways.
dc.language.isoeng
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectTemel Bilimler
dc.subjectTemel Tıp Bilimleri
dc.subjectHistoloji-Embriyoloji
dc.subjectSağlık Bilimleri
dc.subjectTıp
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectHÜCRE BİYOLOJİSİ
dc.titleNFE2L2/NRF2, OGG1, and cytokine responses of human gingival keratinocytes against oxidative insults of various origin
dc.typeMakale
dc.relation.journalMOLECULAR AND CELLULAR BIOCHEMISTRY
dc.contributor.departmentUniversity Of Turku , ,
dc.identifier.volume452
dc.identifier.startpage63
dc.identifier.endpage70
dc.contributor.firstauthorID262208


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