Determination of Phenolic Acids in Atriplex hortensis L. by Novel Solid-Phase Extraction and High-Performance Liquid Chromatography
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A novel offline solid-phase extraction high-performance liquid chromatography method is reported for the determination of phenolic acids in Atriplex hortensis L. Isolation of the analytes was performed by maceration followed by solid-phase extraction with hydrophilic-lipophilic balance cartridges. The chromatography was performed using C18 guard and analytical columns. The mobile phase was 0.08%, aqueous o-phosphoric acid and 40: 60 water/acetonitrile with gradient eluation. The analytes were detected at 254 nm by an ultraviolet detector. Calibration equations were obtained by least squares with a linear dynamic range from 1.00 to 20.00 mg/L. The limits of detection and quantification were from 0.25 to 0.45 and 0.77 to 1.36 mg/L, respectively. The precision was characterized as the repeatability and intermediate precision with samples fortified at low, medium, and high concentrations. The accuracy was evaluated as the recovery. The developed method determined the presence of vanillic acid, syringic acid, and ferulic acid in A. hortensis with good accuracy and precision.
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