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dc.contributor.authorAktas, Zerrin
dc.contributor.authorOncul, Oral
dc.contributor.authorErdem, Fatma
dc.date.accessioned2021-03-03T15:05:33Z
dc.date.available2021-03-03T15:05:33Z
dc.date.issued2018
dc.identifier.citationErdem F., Oncul O., Aktas Z., "Characterization of Resistance Genes and Polymerase Chain Reaction-Based Replicon Typing in Carbapenem-Resistant Klebsiella pneumoniae", MICROBIAL DRUG RESISTANCE, cilt.25, sa.4, ss.551-557, 2018
dc.identifier.issn1076-6294
dc.identifier.othervv_1032021
dc.identifier.otherav_3d1d5955-2ecd-452c-bf25-827ac2bd9e0e
dc.identifier.urihttp://hdl.handle.net/20.500.12627/44990
dc.identifier.urihttps://doi.org/10.1089/mdr.2018.0231
dc.description.abstractBackground: Fifty isolates of Klebsiella pneumoniae isolated from clinical samples between 2012 and 2016 that were found to be resistant to carbapenems were included in this study. Materials and Methods: Resistance genes were investigated by performing PCR. Plasmid typing was performed using PCR-based replicon typing. The clonal relationships between the strains were investigated using pulsed-field gel electrophoresis (PFGE). Results: OXA-48-type carbapenemase genes were detected in 86% (n = 43/50) of K. pneumoniae isolates, whereas NDM-type carbapenemase genes were detected in 14% (n = 7/50) of the isolates. bla(TEM) was detected 60% (n = 30) of the strains, bla(SHV) in 78% (n = 39), bla(CTX-M-1) in 48% (n = 24), and bla(CTX-M-2)-type beta-lactamase in 10% (n = 5). bla(CTX-M-1) and bla(SHV) were concomitantly distributed in 40% (n = 20) of the strains, bla(TEM) and bla(SHV) in 54% (n = 27), bla(TEM), bla(SHV), and bla(CTX-M-1) in 32% (n = 16) and bla(CTX-M-1) and bla(CTX-M-2) in 10% (n = 5). Strain numbers 66, 69, 76, 77, and 78 coproduced carbapenemases, bla(CTX-M-1) and bla(CTX-M-2) in addition to bla(OXA-48) or bla(NDM-1) that were described as hybrid strains. IncR-type replicon was found in 50% (n = 25) of 50 isolates with plasmid typing, whereas IncA/C-type replicon was detected in 40% (n = 20) and IncFIIK-type replicon in 18% (n = 9) of the isolates. Outcomes of the transformation experiments showed that the OXA-48 gene was carried to the receiver cell on FII plasmids. No dominant epidemic clone was detected through PFGE. Conclusion: OXA-48 carbapenemase was found to be the most prevalent type of enzyme in our hospital, and the presence of NDM-1-type carbapenemase-carrying strain and an increase in their rate were detected.
dc.language.isoeng
dc.subjectTemel Eczacılık Bilimleri
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectFarmakoloji ve Toksikoloji
dc.subjectEczacılık
dc.subjectSağlık Bilimleri
dc.subjectFARMAKOLOJİ VE ECZACILIK
dc.subjectMikrobiyoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectİmmünoloji
dc.subjectBULAŞICI HASTALIKLAR
dc.titleCharacterization of Resistance Genes and Polymerase Chain Reaction-Based Replicon Typing in Carbapenem-Resistant Klebsiella pneumoniae
dc.typeMakale
dc.relation.journalMICROBIAL DRUG RESISTANCE
dc.contributor.departmentİstanbul Üniversitesi , İstanbul Tıp Fakültesi , Tıbb Mikrobiyoloji Ad
dc.identifier.volume25
dc.identifier.issue4
dc.identifier.startpage551
dc.identifier.endpage557
dc.contributor.firstauthorID25294


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