dc.contributor.author | Aktas, Zerrin | |
dc.contributor.author | Oncul, Oral | |
dc.contributor.author | Erdem, Fatma | |
dc.date.accessioned | 2021-03-03T15:05:33Z | |
dc.date.available | 2021-03-03T15:05:33Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Erdem F., Oncul O., Aktas Z., "Characterization of Resistance Genes and Polymerase Chain Reaction-Based Replicon Typing in Carbapenem-Resistant Klebsiella pneumoniae", MICROBIAL DRUG RESISTANCE, cilt.25, sa.4, ss.551-557, 2018 | |
dc.identifier.issn | 1076-6294 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_3d1d5955-2ecd-452c-bf25-827ac2bd9e0e | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/44990 | |
dc.identifier.uri | https://doi.org/10.1089/mdr.2018.0231 | |
dc.description.abstract | Background: Fifty isolates of Klebsiella pneumoniae isolated from clinical samples between 2012 and 2016 that were found to be resistant to carbapenems were included in this study. Materials and Methods: Resistance genes were investigated by performing PCR. Plasmid typing was performed using PCR-based replicon typing. The clonal relationships between the strains were investigated using pulsed-field gel electrophoresis (PFGE). Results: OXA-48-type carbapenemase genes were detected in 86% (n = 43/50) of K. pneumoniae isolates, whereas NDM-type carbapenemase genes were detected in 14% (n = 7/50) of the isolates. bla(TEM) was detected 60% (n = 30) of the strains, bla(SHV) in 78% (n = 39), bla(CTX-M-1) in 48% (n = 24), and bla(CTX-M-2)-type beta-lactamase in 10% (n = 5). bla(CTX-M-1) and bla(SHV) were concomitantly distributed in 40% (n = 20) of the strains, bla(TEM) and bla(SHV) in 54% (n = 27), bla(TEM), bla(SHV), and bla(CTX-M-1) in 32% (n = 16) and bla(CTX-M-1) and bla(CTX-M-2) in 10% (n = 5). Strain numbers 66, 69, 76, 77, and 78 coproduced carbapenemases, bla(CTX-M-1) and bla(CTX-M-2) in addition to bla(OXA-48) or bla(NDM-1) that were described as hybrid strains. IncR-type replicon was found in 50% (n = 25) of 50 isolates with plasmid typing, whereas IncA/C-type replicon was detected in 40% (n = 20) and IncFIIK-type replicon in 18% (n = 9) of the isolates. Outcomes of the transformation experiments showed that the OXA-48 gene was carried to the receiver cell on FII plasmids. No dominant epidemic clone was detected through PFGE. Conclusion: OXA-48 carbapenemase was found to be the most prevalent type of enzyme in our hospital, and the presence of NDM-1-type carbapenemase-carrying strain and an increase in their rate were detected. | |
dc.language.iso | eng | |
dc.subject | Temel Eczacılık Bilimleri | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Temel Bilimler | |
dc.subject | Farmakoloji ve Toksikoloji | |
dc.subject | Eczacılık | |
dc.subject | Sağlık Bilimleri | |
dc.subject | FARMAKOLOJİ VE ECZACILIK | |
dc.subject | Mikrobiyoloji | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | İmmünoloji | |
dc.subject | BULAŞICI HASTALIKLAR | |
dc.title | Characterization of Resistance Genes and Polymerase Chain Reaction-Based Replicon Typing in Carbapenem-Resistant Klebsiella pneumoniae | |
dc.type | Makale | |
dc.relation.journal | MICROBIAL DRUG RESISTANCE | |
dc.contributor.department | İstanbul Üniversitesi , İstanbul Tıp Fakültesi , Tıbb Mikrobiyoloji Ad | |
dc.identifier.volume | 25 | |
dc.identifier.issue | 4 | |
dc.identifier.startpage | 551 | |
dc.identifier.endpage | 557 | |
dc.contributor.firstauthorID | 25294 | |