dc.contributor.author | Timirci-Kahraman, Ozlem | |
dc.contributor.author | Sivrikoz, Emre | |
dc.contributor.author | Kurtoglu, Mehmet | |
dc.contributor.author | Zeybek, Umit | |
dc.contributor.author | Ergen, Arzu | |
dc.contributor.author | Aksoy, Murat | |
dc.contributor.author | Yanar, Fatih | |
dc.contributor.author | Isbir, Turgay | |
dc.date.accessioned | 2021-03-03T15:05:04Z | |
dc.date.available | 2021-03-03T15:05:04Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Sivrikoz E., Timirci-Kahraman O., Ergen A., Zeybek U., Aksoy M., Yanar F., Isbir T., Kurtoglu M., "Gene Expression Levels of Elastin and Fibulin-5 According to Differences Between Carotid Plaque Regions", IN VIVO, cilt.29, sa.2, ss.229-235, 2015 | |
dc.identifier.issn | 0258-851X | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_3d0d34b4-d112-4b2d-9a68-ecca29a6923a | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/44957 | |
dc.description.abstract | Aim: The purpose of this study was to investigate the gene expression levels of elastin and fibulin-5 according to differences between carotid plaque regions and to correlate it with clinical features of plaque destabilization. Materials and Methods: The study included 44 endarterectomy specimens available from operated symptomatic carotid artery stenoses. The specimens were separated according to anatomic location: internal carotid artery (ICA), external carotid artery (ECA) and common carotid artery (CCA), and then stored in liquid nitrogen. The amounts of cDNA for elastin and fibulin-5 were determined by Quantitative real-time PCR (Q-RT-PCR). Target gene copy numbers were normalized using hypoxanthine-guanine phosphoribosyltransferase (HPRT1) gene. The delta-delta CT method was applied for relative quantification. Results: Q-RT-PCR data showed that relative fibulin-5 gene expression was increased in ICA plaque regions when compared to CCA regions but not reaching significance (p=0.061). At the same time, no differences were observed in elastin mRNA level between different anatomic plaque regions (p>0.05). Moreover, elastin and fibulin-5 mRNA expression and clinical parameters were compared in ICA plaques versus CCA and ECA regions, respectively. Up-regulation of elastin and fibulin-5 mRNA levels in ICA were strongly correlated with family history of cardiovascular disease when compared to CCA (p<0.05). Up-regulation of fibulin-5 in ICA was significantly associated with diabetes, and elevated triglycerides and very low density lipoprotein (VLDL) when compared to ECA (p<0.05). Conclusion: The clinical significance is the differences between the proximal and distal regions of the lesion, associated with the ICA, CCA and ECA respectively, with increased fibulin-5 in the ICA region. | |
dc.language.iso | eng | |
dc.subject | Tıp | |
dc.subject | Tıbbi Ekoloji ve Hidroklimatoloji | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Dahili Tıp Bilimleri | |
dc.subject | Klinik Tıp (MED) | |
dc.subject | Klinik Tıp | |
dc.subject | TIP, ARAŞTIRMA VE DENEYSEL | |
dc.title | Gene Expression Levels of Elastin and Fibulin-5 According to Differences Between Carotid Plaque Regions | |
dc.type | Makale | |
dc.relation.journal | IN VIVO | |
dc.contributor.department | Ankara Liv Hospital , , | |
dc.identifier.volume | 29 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 229 | |
dc.identifier.endpage | 235 | |
dc.contributor.firstauthorID | 902 | |