dc.contributor.author | Tufan, F. | |
dc.contributor.author | Ucarli, Cüneyt | |
dc.contributor.author | Gurel, F. | |
dc.date.accessioned | 2021-03-03T14:31:14Z | |
dc.date.available | 2021-03-03T14:31:14Z | |
dc.identifier.citation | Ucarli C., Tufan F., Gurel F., "Expression and genomic integration of transgenes after Agrobacterium-mediated transformation of mature barley embryos", GENETICS AND MOLECULAR RESEARCH, cilt.14, ss.1096-1105, 2015 | |
dc.identifier.issn | 1676-5680 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_39fa77a6-148d-45f3-b3ce-35e1efe7275c | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/42970 | |
dc.identifier.uri | https://doi.org/10.4238/2015.february.6.13 | |
dc.description.abstract | Mature embryos in tissue cultures are advantageous because of their abundance and rapid germination, which reduces genomic instability problems. In this study, 2-day-old isolated mature barley embryos were infected with 2 Agrobacterium hypervirulent strains (AGL1 and EHA105), followed by a 3-day period of co-cultivation in the presence of L-cystein amino acid. Chimeric expression of the beta-glucuronidase gene (gusA) directed by a viral promoter of strawberry vein banding virus was observed in coleoptile epidermal cells and seminal roots in 5-day-old germinated seedlings. In addition to varying infectivity patterns in different strains, there was a higher ratio of transient beta-glucuronidase expression in developing coleoptiles than in embryonic roots, indicating the high competency of shoot apical meristem cells in the mature embryo. A total of 548 explants were transformed and 156 plants developed to maturity on G418 media after 18-25 days. We detected transgenes in 74% of the screened plant leaves by polymerase chain reaction, and 49% of these expressed neomycin phosphotransferase II gene following AGL1 transformation. Ten randomly selected T-0 transformants were analyzed using thermal asymmetric interlaced polymerase chain reaction and 24 fragments ranged between 200-600 base pairs were sequenced. Three of the sequences flanked with transferred-DNA showed high similarity to coding regions of the barley genome, including alpha tubulin5, homeobox 1, and mitochondrial 16S genes. We observed 70-200-base pair filler sequences only in the coding regions of barley in this study. | |
dc.language.iso | eng | |
dc.subject | Tıbbi Genetik | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Sitogenetik | |
dc.subject | Temel Bilimler | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Dahili Tıp Bilimleri | |
dc.subject | Tıp | |
dc.subject | GENETİK VE HAYAT | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.title | Expression and genomic integration of transgenes after Agrobacterium-mediated transformation of mature barley embryos | |
dc.type | Makale | |
dc.relation.journal | GENETICS AND MOLECULAR RESEARCH | |
dc.contributor.department | İstanbul Üniversitesi , , | |
dc.identifier.volume | 14 | |
dc.identifier.startpage | 1096 | |
dc.identifier.endpage | 1105 | |
dc.contributor.firstauthorID | 1045294 | |