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dc.contributor.authorAlbeniz, I.
dc.contributor.authorSener, Leyla
dc.contributor.authorKayacan, S.
dc.contributor.authorMelikoglu, G.
dc.contributor.authorKultur, S.
dc.contributor.authorÖztürk, Mahmut Serdar
dc.date.accessioned2021-03-03T14:24:36Z
dc.date.available2021-03-03T14:24:36Z
dc.date.issued2018
dc.identifier.citationKayacan S., Sener L., Melikoglu G., Kultur S., Albeniz I., Öztürk M. S. , "Induction of apoptosis by Centaurea nerimaniae extract in HeLa and MDA-MB-231 cells by a caspase-3 pathway", BIOTECHNIC & HISTOCHEMISTRY, cilt.93, sa.5, ss.311-319, 2018
dc.identifier.issn1052-0295
dc.identifier.othervv_1032021
dc.identifier.otherav_3954fe40-359d-4748-8cb9-0ae3839d342e
dc.identifier.urihttp://hdl.handle.net/20.500.12627/42578
dc.identifier.urihttps://doi.org/10.1080/10520295.2017.1401662
dc.description.abstractWe investigated the cytotoxic and apoptotic effects of a methanol extract of Centaurea nerimaniae, a plant endemic in Turkey, on HeLa and MDA-MB-231 cells. Eight concentrations of C. nerimaniae extract were applied to cells, and cytotoxic effects were measured using the xCELLigence system. The TUNEL assay was used to assess apoptotic cell death and immunohistochemistry was used to determine active caspase-3 using the effective cytotoxic doses of the extract. Doses of 1.42 mg/ml C. nerimaniae inhibited the growth of HeLa cells and 3.67 mg/ml C. nerimaniae inhibited the growth of MDA-MB-231 cells in a dose- and time-dependent manner. The apoptotic indexes for HeLa and MDA-MB-231 cells were increased significantly compared to control groups. Immunohistochemistry showed that the number of caspase-3 immunostained cells increased in the extract treatment groups for both HeLa and MDA-MB-231 cells. In the MDA-MB-231 cell line, caspase-3 immunostaining was observed in nuclei and/or cytoplasm in the extract treated group. Caspase-3 activation was greater in HeLa cells than in MDA-MB-231 cells. We found that the extract of C. nerimaniae had a strong antiproliferative effect and induced apoptosis via caspase-3; MDA-MB-231 cancer cells were more resistant than HeLa cells.
dc.language.isoeng
dc.subjectHistoloji-Embriyoloji
dc.subjectYaşam Bilimleri
dc.subjectBiyoteknoloji
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectTemel Bilimler
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectHÜCRE BİYOLOJİSİ
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMikrobiyoloji
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.titleInduction of apoptosis by Centaurea nerimaniae extract in HeLa and MDA-MB-231 cells by a caspase-3 pathway
dc.typeMakale
dc.relation.journalBIOTECHNIC & HISTOCHEMISTRY
dc.contributor.departmentİstanbul Teknik Üniversitesi , Türk Musikisi Devlet Konservatuarı , Temel Bilimler
dc.identifier.volume93
dc.identifier.issue5
dc.identifier.startpage311
dc.identifier.endpage319
dc.contributor.firstauthorID43747


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