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dc.contributor.authorTek, Cagatay
dc.contributor.authorToydemir, T. F. Seval
dc.contributor.authorSandal, Asiye Izem
dc.contributor.authorBaran, Alper
dc.contributor.authorOzdas, Ozen Banu
dc.contributor.authorEnginler, Sinem Ozlem
dc.contributor.authorErturk, Ezgi
dc.contributor.authorArici, Ramazan
dc.contributor.authorAk, Kemal
dc.contributor.authorKilicarslan, Mehmet Ragip
dc.date.accessioned2021-03-03T14:16:41Z
dc.date.available2021-03-03T14:16:41Z
dc.date.issued2016
dc.identifier.citationEnginler S. O. , Ozdas O. B. , Sandal A. I. , Arici R., Erturk E., Baran A., Toydemir T. F. S. , Tek C., Kilicarslan M. R. , Ak K., "The Effect of Cysteamine and Oviductal Cells in Different Culture Media on the Development of Sheep Embryos", KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.22, sa.1, ss.139-145, 2016
dc.identifier.issn1300-6045
dc.identifier.othervv_1032021
dc.identifier.otherav_38b31a1f-750c-4b91-ad26-27e8a3729cc0
dc.identifier.urihttp://hdl.handle.net/20.500.12627/42197
dc.identifier.urihttps://doi.org/10.9775/kvfd.2015.14115
dc.description.abstractSheep is a very important source of wool, meat and milk all over the world. Oxidative stress during in vitro culture leads to defects in development of gametes and embryos. Several antioxidants such as cysteamine, L-ascorbic acid, beta mercaptoethanol, cysteine, glutathione, proteins, vitamins are used to supplement culture media to counter the oxidative stress. This study was aimed to detect the effect of cysteamine supplementation to the maturation medium and oviductal cell supplementation to culture medium on the subsequent development rates of sheep embryos with the control group. Oocytes were obtained from slaughtered sheep ovaries. Selected oocytes were incubated with or without 100 mu M cysteamine in TCM-199 medium under 38.5-38.8 degrees C 5% CO2 for 23 h. During IVF fresh semen was collected from ram by electroejaculation, they were washed in H-SOF medium and were fertilized in B-SOF medium with oocytes incubated for 18 hours under 38.5-38.8 degrees C 5% CO2, 5% O-2 and 90% N-2. The oocytes were obtained from maturation medium with/without cysteamine (C+,-) and were cultured in SOF or CR1aa media with/without oviductal cells (Ov+,-) and were grouped as; Group Ia: SOF+(C+ Ov-), Group Ib: SOF+(C+ Ov+), Group Ic: SOF+(C-Ov-) Group Id: SOF+(C-Ov+); Group IIa: CR1aa+(C+ Ov-), Group IIb: CR1aa+(C+ Ov+), Group IIc: CR1aa+(C-Ov-), Group IId: CR1aa+(C-Ov+). Embryos were incubated under 38.5-38.8 degrees C 5% CO2, 5% O-2, 90% N-2 in culture medium for 7 days. Embryo developments were observed and recorded daily. GLM procedure found in SPSS packet program was used for statistical analysis in this study. In conclusion, the addition of cysteamine or oviductal cells in vitro culture media found to have any effect in terms of the capacity of reaching to blastocyst stage in SOF or CR1aa media and no statistical difference is detected between groups.
dc.language.isoeng
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectVeteriner Bilimleri
dc.subjectTarımsal Bilimler
dc.subjectSağlık Bilimleri
dc.subjectBitki ve Hayvan Bilimleri
dc.subjectVETERİNERLİK BİLİMLERİ
dc.titleThe Effect of Cysteamine and Oviductal Cells in Different Culture Media on the Development of Sheep Embryos
dc.typeMakale
dc.relation.journalKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
dc.contributor.departmentİstanbul Üniversitesi , ,
dc.identifier.volume22
dc.identifier.issue1
dc.identifier.startpage139
dc.identifier.endpage145
dc.contributor.firstauthorID227602


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