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dc.contributor.authorAkkoc, Tolga
dc.contributor.authorEkici, Aygül
dc.contributor.authorBagis, Haydar
dc.contributor.authorTimur, Metin
dc.contributor.authorAktoprakligil, Digdem
dc.date.accessioned2021-03-03T14:16:15Z
dc.date.available2021-03-03T14:16:15Z
dc.date.issued2010
dc.identifier.citationEkici A., Aktoprakligil D., Timur M., Akkoc T., Bagis H., "The Effect of Linear Versus Circular Vector on Enhanced Green Fluorescent Protein (EGFP) Expression in Transgenic Zebrafish (Danio rerio)", JOURNAL OF ANIMAL AND VETERINARY ADVANCES, cilt.9, sa.8, ss.1232-1236, 2010
dc.identifier.issn1680-5593
dc.identifier.otherav_38a50751-5884-4119-b6db-c8c2f2ee28a0
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/42166
dc.identifier.urihttps://doi.org/10.3923/javaa.2010.1232.1236
dc.description.abstractGreen Fluorescent Protein (GFP) has been used as an indicator of transgene expression in living cells and organisms. In this study, a transgene construct containing the Cyto-Megalo-Virus (CMV) promoter sequences, SV40 polyA signal and the Enhanced Green Fluorescent Protein reporter gene (EGFP) was microinjected into the cytoplasm of one-cell zebrafish embryos. About 65 ng mu L-1 circular and linearized pEGFP-N1 DNA was used in microinjection. Transgenic founders were detected by Polymerase Chain Reaction (PCR), Slot and Southern blots and Reverse-Transcriptase PCR (RT-PCR). EGFP gene expression was detected by inverted fluorescence microscope in F-0 transgenic zebrafish larvae. About 54 and 25 F-0 transgenic zebrafish were obtained after microinjection of linearized and circular gene constructs, respectively. This is the first study for generation of transgenic zebrafish via cytoplasmic DNA microinjection in Turkey. In conclusion, these results indicate that the gene expression efficiency of circular form was higher than the linearized form in F-0 transgenic zebrafish larvae.
dc.language.isoeng
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectVeteriner Bilimleri
dc.subjectVETERİNERLİK BİLİMLERİ
dc.subjectBitki ve Hayvan Bilimleri
dc.subjectSağlık Bilimleri
dc.subjectTarımsal Bilimler
dc.titleThe Effect of Linear Versus Circular Vector on Enhanced Green Fluorescent Protein (EGFP) Expression in Transgenic Zebrafish (Danio rerio)
dc.typeMakale
dc.relation.journalJOURNAL OF ANIMAL AND VETERINARY ADVANCES
dc.contributor.departmentGebze Teknik Üniversitesi , ,
dc.identifier.volume9
dc.identifier.issue8
dc.identifier.startpage1232
dc.identifier.endpage1236
dc.contributor.firstauthorID77019


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