Effects of Semen Extender Supplemented with Bovine Serum Albumin (BSA) on Spermatological Traits of Saanen Buck Semen Stored at+4 degrees C

Abstract

The aim of this study was to investigate the effects of two modified semen diluents on the spermatological parameters of Saanen goat sperm stored at 4 degrees C. Ejaculates were obtained from five Saanen bucks by means of an electro-ejaculator and divided into five aliquots. Then, aliquots of ejaculates were used to create five experimental groups as follows: Group 1 (control), group 2A containing seminal plasma (SP) and bovine serum albumin (BSA; 10 mg/mL(-1)), group 2B containing BSA without SP, group 3A containing egg yolk (EY; 17.00%) and SP and finally group 3B containing EY without SP. All aliquots of semen were extended with Tris-based extender. Spermatological parameters of experimental groups were observed at 0, 6, 12 and 24 h periods during storage at 4 degrees C. Motility of semen in groups 2A and 2B was found to be best values compared to other groups. However, the motility of group 3A at 24 h was 0.00%. In conclusion, it was concluded that the addition of 10 mg/mL(-1) of BSA to Tris-based extender could be useful in the storage of Saanen goat semen regardless of SP at 4 degrees C.