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dc.contributor.authorOzdemir, Osman
dc.contributor.authorUydes-Dogan, Sonmez
dc.contributor.authorTakir, Selcuk
dc.date.accessioned2021-03-03T13:04:07Z
dc.date.available2021-03-03T13:04:07Z
dc.identifier.citationTakir S., Uydes-Dogan S., Ozdemir O., "Retina derived relaxation is mediated by Kir channels and the inhibition of Ca2+ sensitization in isolated bovine retinal arteries", EXPERIMENTAL EYE RESEARCH, cilt.132, ss.240-248, 2015
dc.identifier.issn0014-4835
dc.identifier.othervv_1032021
dc.identifier.otherav_31d317a3-1526-44e4-886f-88632a230b86
dc.identifier.urihttp://hdl.handle.net/20.500.12627/37862
dc.identifier.urihttps://doi.org/10.1016/j.exer.2015.02.003
dc.description.abstractRetinal relaxing factor (RRF) has recently been identified as a novel paracrine regulator of retinal circulation acting differently from well known mediators of the endothelium and the retina. Herein, we aimed to characterize the relaxing mechanism of the retina, i.e. RRF, by evaluating the role of Ca+2-dependent and -independent signaling mechanisms as well as inward rectifier K+ (KO channels. Retinal relaxation was determined by placing a piece of retinal tissue just on top of the precontracted bovine retinal arteries mounted in a wire myograph. The retina produced a complete relaxation response, which display a biphasic character, in depolarized arteries contracted by L-type Ca2+ channel agonist, Bay k 8644. Blockade of L-type Ca2+ channel by nifedipine, inhibition of sarcoplasmic reticulum Ca2+-ATPase by cyclopiazonic acid or removal of extracellular Ca2+ did not influence the prominent relaxation to the retina. Originally, retinal relaxation was found to be unaffected from the inhibition of myosin light chain kinase by ML7, whereas, completely abolished in the presence of myosin light chain phosphatase (MLCP) inhibitor, Calyculin A. Moreover, the inhibition of Rho kinase by its putative inhibitor, Y-27632 displayed comparable relaxant effects to RRF in retinal arteries precontracted either by prostaglandin F25 or K+, and augmented the moderate response to the retina in K+ precontracted arteries. In addition, retinal relaxation was significantly inhibited and lost its biphasic character in the presence of Kir channel blocker, Ba2+. Our results suggested that inhibition of Ca2+ sensitization through the activation of MLCP, possibly via interfering with Rho kinase, and the opening of Kir channels are likely to be involved in the inhibitory influence of RRF on the retinal arteries. (C) 2015 Elsevier Ltd. All rights reserved.
dc.language.isoeng
dc.subjectCerrahi Tıp Bilimleri
dc.subjectGöz Hastalıkları ve Cerrahisi
dc.subjectSağlık Bilimleri
dc.subjectTıp
dc.subjectKlinik Tıp (MED)
dc.subjectKlinik Tıp
dc.subjectOFTALMOLOJİ
dc.titleRetina derived relaxation is mediated by Kir channels and the inhibition of Ca2+ sensitization in isolated bovine retinal arteries
dc.typeMakale
dc.relation.journalEXPERIMENTAL EYE RESEARCH
dc.contributor.departmentİstanbul Üniversitesi , ,
dc.identifier.volume132
dc.identifier.startpage240
dc.identifier.endpage248
dc.contributor.firstauthorID49341


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