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dc.contributor.authorMaurer, Anne-Marie
dc.contributor.authorGezer, Altay
dc.date.accessioned2021-03-03T12:45:58Z
dc.date.available2021-03-03T12:45:58Z
dc.date.issued2013
dc.identifier.citationMaurer A., Gezer A., "Promoting Effects of Heparin on ex vivo Expansion of Megakaryocytopoiesis from Human Cord Blood CD34+Cells", TRANSFUSION MEDICINE AND HEMOTHERAPY, cilt.40, sa.5, ss.344-350, 2013
dc.identifier.issn1660-3796
dc.identifier.otherav_2ffef5e2-e6e6-4181-838d-643e9394eda6
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/36746
dc.identifier.urihttps://doi.org/10.1159/000355519
dc.description.abstractIntroduction: Transfusion of ex vivo expanded megakaryocytes (MKs) has been proposed to sustain platelet recovery after cord blood (CB) hematopoietic stem cell transplantation. In this study, we investigated the effects of heparin on ex vivo colony forming unit-megakaryocytes (CFU-MKs) and MKs expansion from CB CD34+ cells. Methods: CB CD34+ cells were stimulated by a combination of thrombopoietin (TPO), stem cell factor (SCF), Flt3-Ligand (FL), IL-6, and IL-11 supplemented with autologous serum and heparin during 14 days. Expanded cells were analyzed by flow cytometry and cultured in a CFU-MK assay. Results: Compared to control cultures, the 5-factor combination with heparin induced significantly (p = 8N) on day 14; platelets on day 14. The culture-derived platelets were activated upon collagen stimulation. Conclusion: Heparin can significantly enhance the stimulating effects of a combination of TPO, SCF, FL, IL-6, and IL-11 supplemented with autologous serum on CFU-MK, MK, and platelet production from CB CD34+ cells. This expansion system could represent a promising method to generate CFU-MKs and MKs cells for transfusion to sustain platelet reconstitution following CB transplantation.
dc.language.isoeng
dc.subjectİç Hastalıkları
dc.subjectHematoloji
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectSağlık Bilimleri
dc.subjectDahili Tıp Bilimleri
dc.subjectTıp
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectİmmünoloji
dc.subjectKlinik Tıp (MED)
dc.subjectKlinik Tıp
dc.subjectHEMATOLOJİ
dc.titlePromoting Effects of Heparin on ex vivo Expansion of Megakaryocytopoiesis from Human Cord Blood CD34+Cells
dc.typeMakale
dc.relation.journalTRANSFUSION MEDICINE AND HEMOTHERAPY
dc.contributor.departmentMarmara Üniversitesi , ,
dc.identifier.volume40
dc.identifier.issue5
dc.identifier.startpage344
dc.identifier.endpage350
dc.contributor.firstauthorID58392


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