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dc.contributor.authorNurten, Rustem
dc.contributor.authorTurker-Sener, Leyla
dc.contributor.authorBas, Aycan
dc.contributor.authorAlbeniz, Işıl
dc.contributor.authorKalelioglu, Ibrahim
dc.date.accessioned2021-03-03T11:23:09Z
dc.date.available2021-03-03T11:23:09Z
dc.date.issued2012
dc.identifier.citationAlbeniz I., Turker-Sener L., Bas A., Kalelioglu I., Nurten R., "Isolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process", ONCOLOGY LETTERS, cilt.3, sa.1, ss.55-60, 2012
dc.identifier.issn1792-1074
dc.identifier.otherav_27a144e6-20cd-437c-9b21-3f3e42873f76
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/31494
dc.identifier.urihttps://doi.org/10.3892/ol.2011.455
dc.description.abstractThe aim of this study was to investigate changes in primitive hematopoietic cells through CD38 expression, identify the stage at which erythrocyte differentiation CD38 gains activity and the effects of serum factors on this expression by establishing a hematopoietic stem cell system in the erythroid development process. Using an immunomagnetic labeling and separation technique, CD34(+) cells were selected from cord blood. The CD34(+) cells were cultured in a 2 mM L-glutamine-enriched medium containing erythropoietin (Epo), penicillin-streptomycin and stem cell factor (SCF), and were incubated in 5% CO(2) at 37 degrees C. In erythroid development pathways following CD38(+) expression, primitive/progenitor human hematopoietic cells obtained from cord blood were assessed through the erythroid development process in a serum-free medium in the presence of proper SCF and Epo. At the end of the 26-day process, using staining with a Megacult-c staining kit, it was determined that progenitor cells nucleate and differentiate into erythroid cell lines of 8-10 mu m. During the course of this process, we analyzed increases over time in NAD glycohydrolase activity rates using the supernatant liquid samples. Results of co-culture experiments in cell culture studies showed that the stimulating effects of CD38 expression originate from specific serum factors. CD38 expression has been shown to occur at hematopoietic cell sources as well as at a number of differentiation levels. In the proliferation process the possible induction of CD38 through specific serum factors leads us to conclude that it may be involved in proliferation with a physiological task or that it may be involved in an event, such as an apoptotic process.
dc.language.isoeng
dc.subjectİç Hastalıkları
dc.subjectOnkoloji
dc.subjectDahili Tıp Bilimleri
dc.subjectSağlık Bilimleri
dc.subjectTıp
dc.subjectKlinik Tıp (MED)
dc.subjectKlinik Tıp
dc.subjectONKOLOJİ
dc.titleIsolation of hematopoietic stem cells and the effect of CD38 expression during the early erythroid progenitor cell development process
dc.typeMakale
dc.relation.journalONCOLOGY LETTERS
dc.contributor.departmentİstanbul Üniversitesi , ,
dc.identifier.volume3
dc.identifier.issue1
dc.identifier.startpage55
dc.identifier.endpage60
dc.contributor.firstauthorID81465


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