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Isolation and preparation of monoclonal antibody to ovine Immunoglobulin M

dc.contributor.authorTantas, A
dc.contributor.authorMorgan, KL
dc.contributor.authorIlgaz, A
dc.contributor.authorYilmaz, H
dc.date.accessioned2021-03-03T11:11:07Z
dc.date.available2021-03-03T11:11:07Z
dc.date.issued1999
dc.identifier.citationYilmaz H., Tantas A., Ilgaz A., Morgan K., "Isolation and preparation of monoclonal antibody to ovine Immunoglobulin M", TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.23, sa.2, ss.135-140, 1999
dc.identifier.issn1300-0128
dc.identifier.othervv_1032021
dc.identifier.otherav_26799fbb-a805-4084-939b-7b8d12a60ee1
dc.identifier.urihttp://hdl.handle.net/20.500.12627/30747
dc.description.abstractOvine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.
dc.language.isoeng
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectTarımsal Bilimler
dc.subjectSağlık Bilimleri
dc.subjectVeteriner Bilimleri
dc.subjectBitki ve Hayvan Bilimleri
dc.subjectVETERİNERLİK BİLİMLERİ
dc.titleIsolation and preparation of monoclonal antibody to ovine Immunoglobulin M
dc.typeMakale
dc.relation.journalTURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
dc.contributor.department, ,
dc.identifier.volume23
dc.identifier.issue2
dc.identifier.startpage135
dc.identifier.endpage140
dc.contributor.firstauthorID122348


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