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dc.contributor.authorHelps, CR
dc.contributor.authorHarbour, DA
dc.contributor.authorTuran, N
dc.contributor.authorUysal, A
dc.contributor.authorYilmaz, H
dc.date.accessioned2021-03-03T10:34:36Z
dc.date.available2021-03-03T10:34:36Z
dc.date.issued2002
dc.identifier.citationYilmaz H., Helps C., Turan N., Uysal A., Harbour D., "Detection of antibodies to Borna disease virus (BDV) in Turkish horse sera using recombinant p40 - Brief report", ARCHIVES OF VIROLOGY, cilt.147, sa.2, ss.429-435, 2002
dc.identifier.issn0304-8608
dc.identifier.othervv_1032021
dc.identifier.otherav_235f7e05-90ae-431c-979c-185e33127f8f
dc.identifier.urihttp://hdl.handle.net/20.500.12627/28738
dc.identifier.urihttps://doi.org/10.1007/s705-002-8331-8
dc.description.abstractThe nucleoprotein of Borna disease virus (BDV-p40) was produced in a Baculovirus expression system using sf9 cells. The purity and specificity of the recombinant p40 was confirmed by SDS-PAGE and immunoblotting. The recombinant p40 was used in an ELISA to screen horse sera in Turkey. For this, 323 horses from selected cities in the Marmara region of Turkey were examined clinically and serum was collected from each. All horses were clinically healthy except for a few with wounds on the skin. Antibodies to BDV were detected in the sera of 82 (25%) of 323 horse sera. Six sera were selected that had low, medium or high OD values by ELISA and were analysed by Western blotting. All reacted specifically with p40 at a dilution of 1 in 1000. This is the first report of the detection of Borna disease in Turkey and needs further molecular biological investigations to compare the Turkish strains with those strains detected in Europe.
dc.language.isoeng
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectTemel Tıp Bilimleri
dc.subjectViroloji
dc.subjectMikrobiyoloji ve Klinik Mikrobiyoloji
dc.subjectSağlık Bilimleri
dc.subjectTıp
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectİmmünoloji
dc.subjectVİROLOJİ
dc.titleDetection of antibodies to Borna disease virus (BDV) in Turkish horse sera using recombinant p40 - Brief report
dc.typeMakale
dc.relation.journalARCHIVES OF VIROLOGY
dc.contributor.department, ,
dc.identifier.volume147
dc.identifier.issue2
dc.identifier.startpage429
dc.identifier.endpage435
dc.contributor.firstauthorID164179


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