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dc.contributor.authorMetin, Gokhan
dc.contributor.authorDincer, Sensu
dc.contributor.authorMengi, Murat
dc.contributor.authorKarabulut, Aydin
dc.contributor.authorTecellioglu, Fahriye Secil
dc.contributor.authorAKBAŞ, FAHRİ
dc.contributor.authorYILDIZ, MUSTAFA
dc.contributor.authorKumas Kulualp, Meltem
dc.contributor.authorEsrefoglu, Mukaddes
dc.contributor.authorGorucu Yilmaz, Senay
dc.contributor.authorPala, Mukaddes
dc.contributor.authorAltan, Mehmet
dc.contributor.authorSÖNMEZ, OSMAN
dc.date.accessioned2023-10-10T10:42:51Z
dc.date.available2023-10-10T10:42:51Z
dc.date.issued2023
dc.identifier.citationPala M., Gorucu Yilmaz S., Altan M., SÖNMEZ O., Dincer S., Mengi M., Karabulut A., Tecellioglu F. S., AKBAŞ F., YILDIZ M., et al., "Deep phenotyping of miRNAs in exercise-induced cardiac hypertrophy and fibrosis", Journal of Biosciences, cilt.48, sa.4, 2023
dc.identifier.issn0250-5991
dc.identifier.otherav_0aa926d7-dc11-4747-a995-114f084d0d47
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/189463
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85172259707&origin=inward
dc.identifier.urihttps://doi.org/10.1007/s12038-023-00360-4
dc.description.abstractCardiac hypertrophy (CH) is an adaptational enlargement of the myocardium, in exposure to altered stress conditions or in case of injury which can lead to heart failure and death. MicroRNAs (miRNAs) are non-coding RNAs that play a significant role in modulating gene expression. Here, we aimed to identify new miRNAs effective in an experimental CH model and to find an epigenetic biomarker that could demonstrate therapeutic targets responsible for the pathology of heart tissue and serum. In this study, Sprague–Dawley male rats were divided into the training group (TG, n=9) and the control group (CG, n=6). Systolic and diastolic dimensions of the left ventricle and myocardial wall thickness were measured by echocardiography to assess CH. After the exercise program of the rats, miRNA expression measurements and histological analyses were performed. The 25,000 genes in the rat genome were searched using microarray analysis. A total of 128 miRNAs were selected according to the fold change rates, and nine miRNAs were validated for expression analysis. The terminal deoxynucleotidyl transferase dUTP nick (TUNEL) method was used to detect apoptotic cells. Cell proliferation was evaluated by the proliferative cell nuclear antigen (PCNA) method. Necrosis, bleeding, and intercellular edema were detected in TG. The mean histopathological score was higher in TG (p=0.03). There were rarely positive cells for apoptosis of both groups in cardiomyocytes. In the receiver characteristic curve analysis (ROC), the heart tissue rno-miR-290 had an area under the curve (AUC) of 0.920 with 100% sensitivity and 89.90% specificity (p=0.045), rno-miR-194-5p had AUC of 0.940 with 83.33% sensitivity and 100% specificity (p=0.003), and the serum rno-miR-132-3p AUC was 0.880 with 66.67% sensitivity and 100% specificity (p=0.004) in TG. miR-194-5p was used as a therapeutic target for remodeling the cardiac process. While miR-290 contributes to CH as a negative regulator, miR-132 in serum is effective in the pathological and physiological cardiac remodeling process and is a candidate biomarker.
dc.language.isoeng
dc.subjectTarımsal Bilimler
dc.subjectZiraat
dc.subjectYaşam Bilimleri
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectGenel Biyokimya, Genetik ve Moleküler Biyoloji
dc.subjectGenel Tarım ve Biyoloji Bilimleri
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectTarım Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectZİRAAT MÜHENDİSLİĞİ
dc.subjectYaşam Bilimleri (LIFE)
dc.titleDeep phenotyping of miRNAs in exercise-induced cardiac hypertrophy and fibrosis
dc.typeMakale
dc.relation.journalJournal of Biosciences
dc.contributor.departmentGaziantep Üniversitesi , ,
dc.identifier.volume48
dc.identifier.issue4
dc.contributor.firstauthorID4590313


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