dc.contributor.author | İzgi, Demet | |
dc.contributor.author | Akin, Baris | |
dc.contributor.author | Ecder, Tevfik | |
dc.contributor.author | Oguz, Suleyman Rustu | |
dc.contributor.author | Sinangil, Ayse | |
dc.contributor.author | Barlas, Soykan | |
dc.contributor.author | Ciftci, Hayriye Senturk | |
dc.contributor.author | Ulusoy, Ezgi | |
dc.date.accessioned | 2023-05-29T12:56:11Z | |
dc.date.available | 2023-05-29T12:56:11Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Oguz S. R., Sinangil A., Barlas S., Ciftci H. S., Ulusoy E., İzgi D., Ecder T., Akin B., "Correlation of Luminex-Based Single Antigen Based Results With Complement-Dependent Cytotoxicity Crossmatch and Flow Cytometry Crossmatch Results: A Single-Center Experience From Istanbul.", TRANSPLANTATION PROCEEDINGS, cilt.55, sa.1, ss.303-308, 2023 | |
dc.identifier.issn | 0041-1345 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_26174175-6a40-48b0-b90d-445640fb32eb | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/188926 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0041134523000337 | |
dc.identifier.uri | https://doi.org/10.1016/j.transproceed.2023.02.005 | |
dc.description.abstract | ABSTRACTBackgroundThis study aimed to retrospectively investigate the correlation of mean Class I donor-specific antibody (DSA) intensity values detected in Luminex-based techniques with the results of complement-dependent cytotoxicity crossmatch (CDC-XM) and flow cytometry crossmatch (FC-XM) results.MethodsA total of 335 patients with kidney failure and their living donors whose CDC-XM, FC-XM, and single antigen based (SAB) tests were studied between 2018 and 2020 for transplant preparation from living donor candidates were included in the study. Patients were divided into 4 groups according to their mean fluorescence intensity (MFI) values of SAB assay.ResultsAnti-HLA antibodies (class I and/or class II) were detected using SAB in 91.6% patients included in the study (MFI >1000). Class I DSA was positive in 34.8% of patients with anti-HLA antibodies. When CDC-XM and FC-XM results were evaluated in the 4 groups separated according to MFI values, 3 patients with DSA MFI <1000 had negative CDC-XM and T-B-FC-XM results. Of 32 patients with DSA-MFI between 1000 and 3000, 93.75% (n=30) had T-B-FC-XM or CDC-XM-negative results, and 6.25% (n=2) had B-FC-XM-positive results. The CDC-XM, T, and B-FC-XM were negative in all 17 patients with DSA-MFI between 3000 and 5000. Our results showed that MFI >5834 DSA values were significantly correlated with positive T-FC-XM (P< .001), and MFI >6016 values were significantly correlated with positive CDC-XM (P=.002). In addition, MFI values >5000 were associated with both CDC-XM and FC-XM in our study.ConclusionsThe MFI values >5000 correlated with both CDC-XM and FC-XM. | |
dc.language.iso | eng | |
dc.subject | TIP, GENEL & DAHİLİ | |
dc.subject | BİYOLOJİ | |
dc.subject | Tıp | |
dc.subject | Tıbbi Biyoloji | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Temel Tıp Bilimleri | |
dc.subject | Temel Bilimler | |
dc.subject | Genel Sağlık Meslekleri | |
dc.subject | Patofizyoloji | |
dc.subject | Temel Bilgi ve Beceriler | |
dc.subject | Değerlendirme ve Teşhis | |
dc.subject | Dahiliye | |
dc.subject | Aile Sağlığı | |
dc.subject | Tıp (çeşitli) | |
dc.subject | Genel Tıp | |
dc.subject | Multidisipliner | |
dc.subject | Klinik Tıp (MED) | |
dc.subject | Temel Bilimler (SCI) | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Klinik Tıp | |
dc.subject | Doğa Bilimleri Genel | |
dc.subject | Biyoloji ve Biyokimya | |
dc.subject | ÇOK DİSİPLİNLİ BİLİMLER | |
dc.title | Correlation of Luminex-Based Single Antigen Based Results With Complement-Dependent Cytotoxicity Crossmatch and Flow Cytometry Crossmatch Results: A Single-Center Experience From Istanbul. | |
dc.type | Makale | |
dc.relation.journal | TRANSPLANTATION PROCEEDINGS | |
dc.contributor.department | İstanbul Bilim Üniversitesi , Tıp Fakültesi , Temel Tıp Bilimleri Bölümü | |
dc.identifier.volume | 55 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 303 | |
dc.identifier.endpage | 308 | |
dc.contributor.firstauthorID | 4265023 | |