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dc.contributor.authorAkdeniz, Nilgun
dc.contributor.authorÖzsoy, Ceylan
dc.contributor.authorKucukhuseyin, Ozlem
dc.contributor.authorYaylim, Ilhan
dc.contributor.authorOzgen, Ozge
dc.contributor.authorEroglu, Gunes Ozen
dc.contributor.authorKuruca, Serap
dc.date.accessioned2023-02-21T09:05:01Z
dc.date.available2023-02-21T09:05:01Z
dc.date.issued2023
dc.identifier.citationOzgen O., Eroglu G. O., Kucukhuseyin O., Akdeniz N., Özsoy C., Kuruca S., Yaylim I., "Vitamin D increases the efficacy of cisplatin on bladder cancer cell lines", MOLECULAR BIOLOGY REPORTS, cilt.50, sa.1, ss.697-706, 2023
dc.identifier.issn0301-4851
dc.identifier.othervv_1032021
dc.identifier.otherav_279b6e59-42bb-4e70-a265-b01f27cd744a
dc.identifier.urihttp://hdl.handle.net/20.500.12627/187204
dc.identifier.urihttps://avesis.istanbul.edu.tr/api/publication/279b6e59-42bb-4e70-a265-b01f27cd744a/file
dc.identifier.urihttps://doi.org/10.1007/s11033-022-08044-2
dc.description.abstractBackground 1,25(OH)2D3(Calcitriol), which is a broad regulatory molecule, plays a role in changing the efficacy of chemotherapeutic drugs. Cisplatin is one of a current standard chemotherapy regimen for bladder cancer. Increasing the effectiveness of the treatment and reducing the side effects to chemotherapeutics are of great importance in bladder cancer. We aimed to investigate the effect of the combination of cisplatin and calcitriol in order to create a possible advantage in treatment of bladder cancer. Methods T24, ECV-304 and HUVEC cell lines were treated with calcitriol and cisplatin individually and in combination. Dose determination and combination treatments of calcitriol and cisplatin were evaluated using the MTT assay for cytotoxicity analysis on the cells. Annexin V-PI staining method was used for apoptosis determination by flow cytometry. Also the P-gp expression levels were determined by flow cytometry. Results The combination treatment increased the anti-proliferative efficacy compared to the efficacy in cisplatin alone in T24 cells and reduced the cytotoxicity in the HUVEC healthy cells compared to cisplatin alone. Combination treatment achieved significantly higher apoptosis rate in T24 cells compared with the rates in treatment of cisplatin alone. However apoptosis decreased in HUVEC cell line. P-gp ratios were increased in HUVEC and decreased in T24 cells with combination treatment compared to the numbers in the control cells. The rate of apoptosis and P-gp levels showed no significant change in ECV-304 cells. Conclusion Our study revealed that the combination of calcitriol and cisplatin allows the use of cisplatin at lower doses in T24 bladder cancer cell line.
dc.language.isoeng
dc.subjectKlinik Biyokimya
dc.subjectBİYOKİMYA VE MOLEKÜLER BİYOLOJİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectYaşam Bilimleri
dc.subjectSitogenetik
dc.subjectTemel Bilimler
dc.subjectİlaç Keşfi
dc.subjectYapısal Biyoloji
dc.subjectMoleküler Biyoloji
dc.subjectGenel Biyokimya, Genetik ve Moleküler Biyoloji
dc.subjectKanser Araştırmaları
dc.subjectBiyokimya
dc.subjectYaşlanma
dc.subjectBiyokimya, Genetik ve Moleküler Biyoloji (çeşitli)
dc.titleVitamin D increases the efficacy of cisplatin on bladder cancer cell lines
dc.typeMakale
dc.relation.journalMOLECULAR BIOLOGY REPORTS
dc.contributor.departmentİstanbul Teknik Üniversitesi , ,
dc.identifier.volume50
dc.identifier.issue1
dc.identifier.startpage697
dc.identifier.endpage706
dc.contributor.firstauthorID4233913


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