dc.contributor.author | Kizilcay, Gamze | |
dc.contributor.author | Sagirli, Olcay | |
dc.contributor.author | Toker, Sidika Erturk | |
dc.date.accessioned | 2021-12-10T13:14:22Z | |
dc.date.available | 2021-12-10T13:14:22Z | |
dc.identifier.citation | Toker S. E. , Kizilcay G., Sagirli O., "Determination of levofloxacin by HPLC with fluorescence detection in human breast milk", BIOANALYSIS, cilt.13, ss.1063-1070, 2021 | |
dc.identifier.issn | 1757-6180 | |
dc.identifier.other | av_f7ce540f-8de5-43dd-96de-2958dc7fc978 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/175716 | |
dc.identifier.uri | https://doi.org/10.4155/bio-2021-0058 | |
dc.description.abstract | Aim: A new HPLC method with fluorescence detection has been developed and validated for the determination of levofloxacin, one of the fluoroquinolone class antibiotics, in breast milk. Materials & methods: Chromatographic separation was carried out on a reversed phase C18 column with acetonitrile and 10 mM o-phosphoric acid (25:75,v/v) mobile phase composition. Moxifloxacin was used as internal standard and the peaks were detected by fluorescence detection. Results & conclusion: Calibration graph was found linearly within the range of 2.5-500 ng/ml. Limit of detection and limit of quantification were found to be 0.63 and 2.11 ng/ml, respectively. Mean absolute recovery was 96.18%. The developed method has been successfully applied to the determination of levofloxacin in human breast milk taken from two healthy volunteers. | |
dc.language.iso | eng | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Tıp | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Temel Tıp Bilimleri | |
dc.subject | Biyokimya | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Sitogenetik | |
dc.subject | Analitik Kimya | |
dc.subject | Temel Bilimler | |
dc.subject | Biochemistry, Genetics and Molecular Biology (miscellaneous) | |
dc.subject | Clinical Biochemistry | |
dc.subject | Aging | |
dc.subject | Molecular Biology | |
dc.subject | Drug Discovery | |
dc.subject | Biochemistry (medical) | |
dc.subject | Physical Sciences | |
dc.subject | Life Sciences | |
dc.subject | Health Sciences | |
dc.subject | Cancer Research | |
dc.subject | General Biochemistry, Genetics and Molecular Biology | |
dc.subject | Biochemistry | |
dc.subject | Structural Biology | |
dc.subject | Analytical Chemistry | |
dc.subject | Filtration and Separation | |
dc.subject | Chemistry (miscellaneous) | |
dc.subject | General Chemistry | |
dc.subject | BİYOKİMYASAL ARAŞTIRMA YÖNTEMLERİ | |
dc.subject | Biyoloji ve Biyokimya | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | KİMYA, ANALİTİK | |
dc.subject | Kimya | |
dc.subject | Temel Bilimler (SCI) | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.title | Determination of levofloxacin by HPLC with fluorescence detection in human breast milk | |
dc.type | Makale | |
dc.relation.journal | BIOANALYSIS | |
dc.contributor.department | İstanbul Üniversitesi , Eczacılık Fakültesi , Temel Eczacılık Bilimleri Bölümü | |
dc.identifier.volume | 13 | |
dc.identifier.startpage | 1063 | |
dc.identifier.endpage | 1070 | |
dc.contributor.firstauthorID | 2656026 | |