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dc.contributor.authorInce, Bahar
dc.contributor.authorİnce, Orhan
dc.contributor.authorAydin, Sevcan
dc.date.accessioned2021-12-10T13:08:04Z
dc.date.available2021-12-10T13:08:04Z
dc.identifier.citationAydin S., Ince B., İnce O., "Development of antibiotic resistance genes in microbial communities during long-term operation of anaerobic reactors in the treatment of pharmaceutical wastewater", WATER RESEARCH, cilt.83, ss.337-344, 2015
dc.identifier.issn0043-1354
dc.identifier.othervv_1032021
dc.identifier.otherav_f0950778-8e42-4a24-bb4c-5511cbb0c615
dc.identifier.urihttp://hdl.handle.net/20.500.12627/175479
dc.identifier.urihttps://doi.org/10.1016/j.watres.2015.07.007
dc.description.abstractBiological treatment processes offer the ideal conditions in which a high diversity of microorganisms can grow and develop. The wastewater produced during these processes is contaminated with antibiotics and, as such, they provide the ideal setting for the acquisition and proliferation of antibiotic resistance genes (ARGs). This research investigated the occurrence and variation in the ARGs found during the one-year operation of the anaerobic sequencing batch reactors (SBRs) used to treat pharmaceutical wastewater that contained combinations of sulfamethoxazole-tetracycline-erythromycin (STE) and sulfamethoxazole-tetracycline (ST). The existence of eighteen ARGs encoding resistance to sulfamethoxazole sul2, sul3), erythromycin (ermA, ermF, ermB, msrA, ereA), tetracycline (tetA, tetB, tetC, tetD, tetE, tetM, tetS, tetQ tetW, tetX) and class I integron gene (intI 1) in the STE and ST reactors was investigated by quantitative real-time PCR. Due to the limited availability of primers to detect ARGs, Illumina sequencing was also performed on the sludge and effluent of the STE and ST reactors. Although there was good reactor performance in the SBRs, which corresponds to min 80% COD removal efficiency, tetA, tetB, sul1, sul2 and ermB genes were among those ARGs detected in the effluent from STE and ST reactors. A comparison of the ARGs acquired from the STE and ST reactors revealed that the effluent from the STE reactor had a higher number of ARGs than that from the ST reactor; this could be due to the synergistic effects of erythromycin. According to the expression of genes results, microorganisms achieve tetracycline and erythromycin resistance through a combination of three mechanisms: efflux pumping protein, modification of the antibiotic target and modifying enzymes. There was also a significant association between the presence of the class 1 integron and sulfamethoxazole resistance genes. (C) 2015 Elsevier Ltd. All rights reserved.
dc.language.isoeng
dc.subjectAquatic Science
dc.subjectWaste Management and Disposal
dc.subjectGeneral Engineering
dc.subjectPollution
dc.subjectWater Science and Technology
dc.subjectEnvironmental Chemistry
dc.subjectNature and Landscape Conservation
dc.subjectEngineering (miscellaneous)
dc.subjectEnvironmental Science (miscellaneous)
dc.subjectPhysical Sciences
dc.subjectLife Sciences
dc.subjectEnvironmental Engineering
dc.subjectMÜHENDİSLİK, ÇEVRE
dc.subjectMühendislik
dc.subjectMühendislik, Bilişim ve Teknoloji (ENG)
dc.subjectÇEVRE BİLİMLERİ
dc.subjectÇevre / Ekoloji
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectSU KAYNAKLARI
dc.subjectTarımsal Bilimler
dc.subjectZiraat
dc.subjectToprak ve Bitki Besleme
dc.subjectToprak ve Su Muhafazası ve Amenajmanı
dc.subjectHavza Yönetimi
dc.subjectÇevre Mühendisliği
dc.subjectMühendislik ve Teknoloji
dc.titleDevelopment of antibiotic resistance genes in microbial communities during long-term operation of anaerobic reactors in the treatment of pharmaceutical wastewater
dc.typeMakale
dc.relation.journalWATER RESEARCH
dc.contributor.departmentİstanbul Teknik Üniversitesi , ,
dc.identifier.volume83
dc.identifier.startpage337
dc.identifier.endpage344
dc.contributor.firstauthorID2605107


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