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Comparison of parthenogenetic oocyte activation in different mouse strains on in vitro development rate and quality

dc.contributor.authorGül, Şeref
dc.contributor.authorTaşkın, Ali Cihan
dc.contributor.authorÇağlar Erkal, Kübra
dc.contributor.authorKocabay, Ahmet
dc.date.accessioned2021-12-10T10:54:59Z
dc.date.available2021-12-10T10:54:59Z
dc.date.issued2020
dc.identifier.citationTaşkın A. C. , Kocabay A., Gül Ş., Çağlar Erkal K., "Comparison of parthenogenetic oocyte activation in different mouse strains on in vitro development rate and quality", JOURNAL OF THE TURKISH VETERINARY MEDICAL SOCIETY, cilt.92, sa.1, ss.41-47, 2020
dc.identifier.otherav_5bfdf05e-55e8-453f-8cdd-ec864f8ebcdf
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/170833
dc.identifier.urihttps://doi.org/10.33188/vetheder.789268
dc.description.abstractThe aim of our research is to investigate the effects of parthenogenetic activation on in vitro embryo development rates in different mouse strains. B6CBAF1, C57BL/6j, and B6D2F1 mouse strains were used in this study. Superovulated mice were sacrificed and oocytes were obtained 14 hours after the human chorionic gonadotrophin (hCG) injection and the parthenogenetic activation started 18 hours after hCG injection. The oocytes were activated for 6 hours in 10 mM SrCl2 + 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing Ca2+ free Chatot Ziomek Brinster (CZB) activation medium. After this, further incubation was performed for two hours in an incubator at 37 °C and 5% CO2 in embryo culturing medium + TSA. Finally, embryos were cultured for 120 hours. Parthenogenetic activation success of the B6D2F1 mouse strain was found to be higher than C57BL/6j and B6CBAF1 strains
dc.language.isotur
dc.subjectVeteriner Dölerme ve Suni Tohumlama
dc.subjectBitki ve Hayvan Bilimleri
dc.subjectVeterinary (miscellaneous)
dc.subjectGeneral Veterinary
dc.subjectEquine
dc.subjectHealth Sciences
dc.subjectVETERİNERLİK BİLİMLERİ
dc.subjectSağlık Bilimleri
dc.subjectVeteriner Bilimleri
dc.subjectKlinik Bilimler
dc.subjectKlinik Tıp (MED)
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.titleComparison of parthenogenetic oocyte activation in different mouse strains on in vitro development rate and quality
dc.typeMakale
dc.relation.journalJOURNAL OF THE TURKISH VETERINARY MEDICAL SOCIETY
dc.contributor.department, ,
dc.identifier.volume92
dc.identifier.issue1
dc.identifier.startpage41
dc.identifier.endpage47
dc.contributor.firstauthorID2748790


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