dc.contributor.author | Nurten, A. | |
dc.contributor.author | Ozerman-Edis, Bilge | |
dc.contributor.author | Kara, I | |
dc.date.accessioned | 2021-12-10T10:18:09Z | |
dc.date.available | 2021-12-10T10:18:09Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Ozerman-Edis B., Nurten A., Kara I., "Blockage of Voltage-Dependent Calcium Channels Affects Twitch Response of Rat Skeletal Muscle", NEUROCHEMICAL JOURNAL, cilt.15, sa.2, ss.154-158, 2021 | |
dc.identifier.issn | 1819-7124 | |
dc.identifier.other | av_371a84ab-1c2e-47c1-9f1d-df70f3bbb8fb | |
dc.identifier.other | vv_1032021 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/169614 | |
dc.identifier.uri | https://doi.org/10.1134/s1819712421020136 | |
dc.description.abstract | Neurotransmitter release is controlled by calcium (Ca2+) entry into motor nerve terminals (MNTs) through voltage-dependent calcium channels (VDCCs). Upon Ca2+ influx, acetylcholine is released starting downstream processes causing muscle contraction. Co-release of acetylcholine and glutamate from MNTs has been reported. Ca2+ influx through N-methyl-D-aspartate (NMDA) receptors on postsynaptic site is evident. In this study we aimed to observe nerve-evoked and directly-elicited twitch responses in the presence of VDCCs inhibitors without blocking NMDA receptors. We elicited contractions with nerve-evoked (0.1 Hz; 0.3 ms) and direct (0.1 Hz; 3 ms) electrical stimulations at rat hemidiaphragm preparations by using some VDCC toxins and drugs. We evaluated their effects at 15 minutes of application. P/Q-type VDCC blocker omega-conotoxin MVIIC (25 nM) suppressed the nerve-evoked and direct stimulation contractions relative to their initial value by 50 and 40%, respectively. N-type VDCC inhibitor omega-conotoxin GVIA (170 nM) had no effect on both stimulation contractions. P-type VDCC blocker omega-agatoxin IVA (10 nM) completely blocked the nerve-evoked stimulation contractions, whereas the direct stimulation contractions maintained the same values as baseline. L-type VDCC blockers verapamil (75 mu M) and diltiazem (75 mu M) depressed both nerve-evoked and direct stimulation contractions. Ethanol (650 mM) blocked nearly 100% of the contractions by nerve-evoked stimulation, meanwhile 45.5% of direct stimulation. Our finding may suggest the Ca2+ influx localization matters for decoding the neural information for the contractile force generation. | |
dc.language.iso | eng | |
dc.subject | General Neuroscience | |
dc.subject | Neuroscience (miscellaneous) | |
dc.subject | Sensory Systems | |
dc.subject | Human-Computer Interaction | |
dc.subject | Physical Sciences | |
dc.subject | Life Sciences | |
dc.subject | Cellular and Molecular Neuroscience | |
dc.subject | Cognitive Neuroscience | |
dc.subject | Developmental Neuroscience | |
dc.subject | Temel Bilimler | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Sinirbilim ve Davranış | |
dc.subject | NEUROSCIENCES | |
dc.title | Blockage of Voltage-Dependent Calcium Channels Affects Twitch Response of Rat Skeletal Muscle | |
dc.type | Makale | |
dc.relation.journal | NEUROCHEMICAL JOURNAL | |
dc.contributor.department | İstanbul Yeni Yüzyıl Üniversitesi , , | |
dc.identifier.volume | 15 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 154 | |
dc.identifier.endpage | 158 | |
dc.contributor.firstauthorID | 2695979 | |