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dc.contributor.authorManefield, Michael J.
dc.contributor.authorErtan, Haluk
dc.contributor.authorHolland, Sophie
dc.contributor.authorLee, Matthew
dc.contributor.authorMontgomery, Kate
dc.date.accessioned2021-03-02T15:45:21Z
dc.date.available2021-03-02T15:45:21Z
dc.identifier.citationHolland S., Ertan H., Montgomery K., Manefield M. J. , Lee M., "Novel dichloromethane-fermenting bacteria in the Peptococcaceae family", ISME JOURNAL, 2021
dc.identifier.issn1751-7362
dc.identifier.othervv_1032021
dc.identifier.otherav_30e56be9-4a3d-4d13-969a-0683c82fc836
dc.identifier.urihttp://hdl.handle.net/20.500.12627/1658
dc.identifier.urihttps://doi.org/10.1038/s41396-020-00881-y
dc.description.abstractDichloromethane (DCM; CH2Cl2) is a toxic groundwater pollutant that also has a detrimental effect on atmospheric ozone levels. As a dense non-aqueous phase liquid, DCM migrates vertically through groundwater to low redox zones, yet information on anaerobic microbial DCM transformation remains scarce due to a lack of cultured organisms. We report here the characterisation of DCMF, the dominant organism in an anaerobic enrichment culture (DFE) capable of fermenting DCM to the environmentally benign product acetate. Stable carbon isotope experiments demonstrated that the organism assimilated carbon from DCM and bicarbonate via the Wood-Ljungdahl pathway. DCMF is the first anaerobic DCM-degrading population also shown to metabolise non-chlorinated substrates. It appears to be a methylotroph utilising the Wood-Ljungdahl pathway for metabolism of methyl groups from methanol, choline, and glycine betaine. The flux of these substrates from subsurface environments may either directly (DCM, methanol) or indirectly (choline, glycine betaine) affect the climate. Community profiling and cultivation of cohabiting taxa in culture DFE without DCMF suggest that DCMF is the sole organism in this culture responsible for substrate metabolism, while the cohabitants persist via necromass recycling. Genomic and physiological evidence support placement of DCMF in a novel genus within the Peptococcaceae family, 'Candidatus Formimonas warabiya'.
dc.language.isoeng
dc.subjectTarımsal Bilimler
dc.subjectEKOLOJİ
dc.subjectEcology
dc.subjectÇevre / Ekoloji
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectMikrobiyoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectÇevre Mühendisliği
dc.subjectÇevre Teknolojisi
dc.subjectEkoloji ve Kirlenme
dc.subjectYaşam Bilimleri
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectEcology, Evolution, Behavior and Systematics
dc.subjectEcological Modeling
dc.subjectPhysical Sciences
dc.subjectLife Sciences
dc.titleNovel dichloromethane-fermenting bacteria in the Peptococcaceae family
dc.typeMakale
dc.relation.journalISME JOURNAL
dc.contributor.departmentUniversity of New South Wales Sydney , ,
dc.contributor.firstauthorID2513595


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