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dc.contributor.authorYildizhan, Yasemin
dc.contributor.authorDalyan, Eda
dc.contributor.authorYuksel, Bayram
dc.contributor.authorMemon, Abdul Razaque
dc.contributor.authorCevher-Keskin, Birsen
dc.date.accessioned2021-03-06T20:50:48Z
dc.date.available2021-03-06T20:50:48Z
dc.date.issued2019
dc.identifier.citationCevher-Keskin B., Yildizhan Y., Yuksel B., Dalyan E., Memon A. R. , "Characterization of differentially expressed genes to Cu stress in Brassica nigra by Arabidopsis genome arrays", ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, cilt.26, sa.1, ss.299-311, 2019
dc.identifier.issn0944-1344
dc.identifier.otherav_fc08d7e3-c60d-4be3-87e3-5195e49fd990
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/164961
dc.identifier.urihttps://doi.org/10.1007/s11356-018-3577-7
dc.description.abstractPhytoremediation is an efficient and promising cleanup technology to extract or inactivate heavy metals and several organic and inorganic pollutants from soil and water. In this study, different Brassica nigra L. ecotypes, including Diyarbakr, collected from mining areas were exposed to different concentrations of copper and harvested after 72h of Cu stress for the assessment of phytoremediation capacity. The Diyarbakr ecotype was called as metallophyte because of surviving at 500M Cu. To better understand Cu stress mechanism, ArabidopsisATH1 genome array was used to compare the gene expression in root and shoot tissues of B. nigra under 25M Cu. The response to Cu was much stronger in roots (88 genes showing increased or decreased mRNA levels) than in leaf tissues (24 responding genes). These genes were classified into the metal transport and accumulation-related genes, signal transduction and metabolism-related genes, and transport facilitation genes. Glutathione pathway-related genes (-ECS, PC, etc.) mRNAs were identified as differentially expressed in root and shoot tissues. QRT-PCR validation experiments showed that -ECS and PC expression was upregulated in the shoot and leaf tissues of the 100M Cu-subjected B. nigra-tolerant ecotype. This is the first study showing global expression profiles in response to Cu stress in B. nigra by Arabidopsis genome array. This work presented herein provides a well-illustrated insight into the global gene expression to Cu stress response in plants, and identified genes from microarray data will serve as molecular tools for the phytoremediation applications in the future.
dc.language.isoeng
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectÇevre Mühendisliği
dc.subjectÇEVRE BİLİMLERİ
dc.subjectÇevre / Ekoloji
dc.subjectTarımsal Bilimler
dc.subjectMühendislik ve Teknoloji
dc.titleCharacterization of differentially expressed genes to Cu stress in Brassica nigra by Arabidopsis genome arrays
dc.typeMakale
dc.relation.journalENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
dc.contributor.departmentTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) , ,
dc.identifier.volume26
dc.identifier.issue1
dc.identifier.startpage299
dc.identifier.endpage311
dc.contributor.firstauthorID85910


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