dc.contributor.author | ÖZDEMİR, ELİF | |
dc.contributor.author | Ulu, Sevgi Tatar | |
dc.date.accessioned | 2021-03-06T19:50:17Z | |
dc.date.available | 2021-03-06T19:50:17Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | ÖZDEMİR E., Ulu S. T. , "Highly sensitive HPLC method for the determination of galantamine in human plasma and urine through derivatization with dansyl chloride using fluorescence detector", LUMINESCENCE, cilt.32, ss.1145-1149, 2017 | |
dc.identifier.issn | 1522-7235 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.other | av_f839a7bf-7bd4-4348-a4e7-b2a0369b62a7 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/162619 | |
dc.identifier.uri | https://doi.org/10.1002/bio.3301 | |
dc.description.abstract | A new method based on fluorescence derivatization with 5-(dimethylamino) naphthalene-1-sulfonyl chloride (dansyl chloride) was developed for the quantitative determination of galantamine in human plasma and urine using high-performance liquid chromatography. The reaction between galantamine and dansyl chloride was optimally realized in 30min at room temperature and pH10.5, with a reagent to galantamine molar ratio of 2.13. The derivative was extracted with dichloromethane, and the extract was dried under a nitrogen stream and dissolved in the mobile phase. Chromatographic analysis was performed with an Inertsil C-18 column and a mobile phase comprising 40% acetonitrile and 60% 10mM o-phosphoric acid, 1.2ml/min. The injection volume was 20l. The derivatives were detected with a fluorescence detector (excitation 375nm/emission 537nm). The retention time for the dansyl derivative of galantamine was 16.8min. Linearity was observed between 125 and 2000ng/ml in water, urine and plasma. The limit of detection and limit of quantification for the developed method were 6.27-70.99 and 18.81-212.97ng/ml, respectively. Per cent recovery was calculated as 95.15 for urine and 95.78 for plasma. Interday repeatability values for urine and plasma samples (n=6) at three different concentrations were calculated as a per cent relative standard deviation of 0.24-0.59 and 0.35-0.56. The corresponding per cent relative standard deviation values for intraday repeatability were 0.13-0.51 and 0.04-0.15, respectively. | |
dc.language.iso | eng | |
dc.subject | Temel Bilimler | |
dc.subject | Sitogenetik | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | Yaşam Bilimleri | |
dc.subject | Yaşam Bilimleri (LIFE) | |
dc.subject | Moleküler Biyoloji ve Genetik | |
dc.subject | BİYOKİMYA VE MOLEKÜLER BİYOLOJİ | |
dc.title | Highly sensitive HPLC method for the determination of galantamine in human plasma and urine through derivatization with dansyl chloride using fluorescence detector | |
dc.type | Makale | |
dc.relation.journal | LUMINESCENCE | |
dc.contributor.department | İstanbul Yeni Yüzyıl Üniversitesi , Eczacılık Fakültesi , -- | |
dc.identifier.volume | 32 | |
dc.identifier.issue | 7 | |
dc.identifier.startpage | 1145 | |
dc.identifier.endpage | 1149 | |
dc.contributor.firstauthorID | 247259 | |