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dc.contributor.authorUlu, Sevgi
dc.date.accessioned2021-03-06T12:36:57Z
dc.date.available2021-03-06T12:36:57Z
dc.date.issued2007
dc.identifier.citationUlu S., "Development of an HPLC method for the determination of mexiletine in human plasma and urine by solid-phase extraction", TALANTA, cilt.72, ss.1172-1177, 2007
dc.identifier.issn0039-9140
dc.identifier.othervv_1032021
dc.identifier.otherav_f4b3590d-8512-4bc1-baac-b5bf6f8bcef7
dc.identifier.urihttp://hdl.handle.net/20.500.12627/160398
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2007.01.008
dc.description.abstractA sensitive and specific high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of mexiletine (MEX) in human plasma and urine. It uses solid-phase extraction (SPE) followed by an automated reversed-phase HPLC with a pre-column derivatization with 4-chloro-7-nitrobenzofurazan (NBD-Cl) and UV-vis Absorbance detection. The process was set as: the UV-vis Absorbance wavelength was set at 458 nm. Chromatographic separation was performed on a Phenomenex-C-18 Column (Aqua, 150 mm x 4.6 mm i.d. with 5 Lm particle size) with the mobile phase consisting of acetonitrile and water (80:20, v/v), and the flow rate was set at 1.0 mL min(-1). Calibration of the overall analytical procedure gave a linear signal (r > 0.9998) over a MEX concentration range of 0.2-2.0 mu g mL(-1) in human plasma and urine. The detection limit in plasma and urine was 0.1 mu g mL(-1). Intra- and inter-day precision of the assay at three concentrations within this range were 0.31-2.50%. The high specificity and sensitivity have been achieved by this fast method (total run-time <6 min). The method has been successfully validated in human plasma and urine and it has been shown to be precise, accurate and reliable. (C) 2007 Elsevier B.V. All rights reserved.
dc.language.isoeng
dc.subjectTemel Bilimler (SCI)
dc.subjectKİMYA, ANALİTİK
dc.subjectKimya
dc.subjectAnalitik Kimya
dc.subjectTemel Bilimler
dc.titleDevelopment of an HPLC method for the determination of mexiletine in human plasma and urine by solid-phase extraction
dc.typeMakale
dc.relation.journalTALANTA
dc.contributor.departmentİstanbul Üniversitesi , Eczacılık Fakültesi , Temel Eczacılık Bilimleri Bölümü
dc.identifier.volume72
dc.identifier.issue3
dc.identifier.startpage1172
dc.identifier.endpage1177
dc.contributor.firstauthorID61753


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