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dc.contributor.authorAktan, Gulsan
dc.contributor.authorKucukgergin, Canan
dc.contributor.authorUysal, Mujdat
dc.contributor.authorKadioglu, Ates
dc.contributor.authorKocak-Toker, Necla
dc.contributor.authorErata, Gul Ozdemirler
dc.date.accessioned2021-03-05T19:54:12Z
dc.date.available2021-03-05T19:54:12Z
dc.date.issued2013
dc.identifier.citationErata G. O. , Kucukgergin C., Aktan G., Kadioglu A., Uysal M., Kocak-Toker N., "Is thioredoxin reductase involved in the defense against DNA fragmentation in varicocele?", ASIAN JOURNAL OF ANDROLOGY, cilt.15, ss.518-522, 2013
dc.identifier.issn1008-682X
dc.identifier.otherav_d16cae61-2c7e-4461-a0eb-4110e3c32b64
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/138430
dc.identifier.urihttps://doi.org/10.1038/aja.2013.9
dc.description.abstractWe aimed to investigate the role of thioredoxin reductase (TR) and inducible heat shock protein 70 (iHsp70) and their relationship with sperm quality in varicocele (VAR) patients. Semen samples were obtained from 16 subfertile men diagnosed as VAR and 10 fertile men who applied to the Andrology Laboratory of Istanbul Medical Faculty of Istanbul University. The sperm TR and iHsp 70 expression levels were determined using Western blot analysis. The TR activity of the sperm was assayed spectrophometrically. The sperm quality was evaluated both by conventional sperm analysis and by a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) technique that assayed DNA-fragmented spermatozoa in semen samples. The percentage of TUNEL-positive spermatozoa in the VAR group (16.3%+/- 5.6%) was higher than that in the fertile group (5.5%+/- 1.9%). Significant inverse correlations were detected between the percentage of TUNEL-positive cells and both the concentration (r=-0.609; P=0.001) and motility (r=-0.550; P=0.004) of spermatozoa. Both the TR expression and activity were increased significantly in the VAR group (U=22.0; P=0.001 and U=33.5; P=0.012, respectively) as analyzed using the Mann-Whitney U Wilcoxon rank sum W test. Furthermore, significant positive correlations were found between TR expression and activity (r=0.406; P=0.040) and between TR expression and the percentage of TUNEL-positive cells (r=0.665; P=0.001). Sperm iHsp70 expression did not differ between the VAR and fertile groups. In conclusion, increased sperm TR expression might be a defense mechanism against apoptosis in the spermatozoa of men with VAR.
dc.language.isoeng
dc.subjectİç Hastalıkları
dc.subjectEndokrinoloji ve Metabolizma Hastalıkları
dc.subjectNefroloji
dc.subjectTıp
dc.subjectDahili Tıp Bilimleri
dc.subjectSağlık Bilimleri
dc.subjectENDOKRİNOLOJİ VE METABOLİZMA
dc.subjectÜROLOJİ VE NEFROLOJİ
dc.subjectKlinik Tıp (MED)
dc.subjectKlinik Tıp
dc.subjectANDROLOJİ
dc.titleIs thioredoxin reductase involved in the defense against DNA fragmentation in varicocele?
dc.typeMakale
dc.relation.journalASIAN JOURNAL OF ANDROLOGY
dc.contributor.departmentİstanbul Üniversitesi , ,
dc.identifier.volume15
dc.identifier.issue4
dc.identifier.startpage518
dc.identifier.endpage522
dc.contributor.firstauthorID16898


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