Basit öğe kaydını göster

dc.contributor.authorGuzel, E.
dc.contributor.authorBolgen, N.
dc.contributor.authorYang, Y.
dc.contributor.authorKORKUSUZ, PETEK
dc.contributor.authorEl Haj, A. J.
dc.contributor.authorPiskin, E.
dc.date.accessioned2021-03-05T14:54:40Z
dc.date.available2021-03-05T14:54:40Z
dc.date.issued2011
dc.identifier.citationBolgen N., Yang Y., KORKUSUZ P., Guzel E., El Haj A. J. , Piskin E., "3D ingrowth of bovine articular chondrocytes in biodegradable cryogel scaffolds for cartilage tissue engineering", JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, cilt.5, ss.770-779, 2011
dc.identifier.issn1932-6254
dc.identifier.otherav_b963dda2-ed77-4706-ab52-e99b22ef2eba
dc.identifier.othervv_1032021
dc.identifier.urihttp://hdl.handle.net/20.500.12627/123348
dc.identifier.urihttps://doi.org/10.1002/term.375
dc.description.abstractA feasibility study was undertaken to examine the potential of biodegradable HEMA-lactate-dextran (HEMA-LLA-D)-based cryogels as scaffolds for cartilage tissue engineering. This was a preliminary in vitro study giving essential information on the biocompatibility of cryogels with cartilage cells. HEMA-lactate (HEMA-LLA) and HEMA-LLA-D were synthesized and characterized by different techniques. Cryogel scaffolds with supermacroporous structures were produced by cryogenic treatment of these macromers. Chondrocytes obtained from bovine articular cartilage were seeded onto cylindrical cryogels and cultured. The samples were examined by several microcopical techniques for cell viability and morphological analyses were performed at two culture points. Histological study of the constructs revealed the cells' growth on the surface and within the scaffolds. Confocal microscopical images demonstrated that the majority of live vs. dead cells had been attached to and integrated with the pores of the scaffold. SEM analysis showed round to oval-shaped chondrocytic cells interconnected with each other by communicating junctions. The chondrocytes rapidly proliferated in the cryogels, manifesting that they fully covered the scaffold surface after 9 days and almost filled the spaces in the pores of the scaffold after 15 days of culture. Chondrocytes secreted significant amount of extracellular matrix in the scaffolds and exhibited highly interconnective morphology. Light and transmission electron microscopy revealed groups of active cartilage cells closely apposed to the cryogel. We concluded that cryogel scaffolds could be excellent candidates for cartilage tissue regeneration with their extraordinary properties, including soft, elastic nature, highly open interconnected pore structure and very rapid, controllable swellability. Copyright (C) 2010 John Wiley & Sons, Ltd.
dc.language.isoeng
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectMühendislik ve Teknoloji
dc.subjectTemel Bilimler
dc.subjectHÜCRE VE DOKU MÜHENDİSLİĞİ
dc.subjectBiyoloji ve Biyokimya
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectMikrobiyoloji
dc.subjectHÜCRE BİYOLOJİSİ
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectMÜHENDİSLİK, BİYOMEDİKSEL
dc.subjectMühendislik
dc.subjectMühendislik, Bilişim ve Teknoloji (ENG)
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectHistoloji-Embriyoloji
dc.subjectBiyomedikal Mühendisliği
dc.subjectYaşam Bilimleri
dc.subjectBiyoteknoloji
dc.title3D ingrowth of bovine articular chondrocytes in biodegradable cryogel scaffolds for cartilage tissue engineering
dc.typeMakale
dc.relation.journalJOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
dc.contributor.departmentMersin Üniversitesi , ,
dc.identifier.volume5
dc.identifier.issue10
dc.identifier.startpage770
dc.identifier.endpage779
dc.contributor.firstauthorID75279


Bu öğenin dosyaları:

DosyalarBoyutBiçimGöster

Bu öğe ile ilişkili dosya yok.

Bu öğe aşağıdaki koleksiyon(lar)da görünmektedir.

Basit öğe kaydını göster