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dc.contributor.authorBaskan, Kevser Sozgen
dc.contributor.authorYıldız, Leyla
dc.contributor.authorCelik, S. Esin
dc.contributor.authorTutem, Esma
dc.contributor.authorGuclu, Kubilay
dc.contributor.authorKaraman, Seyda
dc.contributor.authorApak, Resat
dc.contributor.authorBaki, Sefa
dc.contributor.authorErcag, Erol
dc.contributor.authorOzyurek, Mustafa
dc.date.accessioned2021-03-05T12:47:07Z
dc.date.available2021-03-05T12:47:07Z
dc.date.issued2011
dc.identifier.citationOzyurek M., Guclu K., Tutem E., Baskan K. S. , Ercag E., Celik S. E. , Baki S., Yıldız L., Karaman S., Apak R., "A comprehensive review of CUPRAC methodology", ANALYTICAL METHODS, cilt.3, ss.2439-2453, 2011
dc.identifier.issn1759-9660
dc.identifier.othervv_1032021
dc.identifier.otherav_aee45dc2-d019-4fc7-8b7f-ed1dd58d4777
dc.identifier.urihttp://hdl.handle.net/20.500.12627/116664
dc.identifier.urihttps://doi.org/10.1039/c1ay05320e
dc.description.abstractMeasuring the antioxidant activity/capacity levels of food and biological fluids is carried out for the meaningful comparison of the antioxidant content of foodstuffs and for the diagnosis and treatment of oxidative stress-associated diseases in clinical biochemistry. Current literature clearly states that there is no widely adopted/accepted "total antioxidant parameter" as a nutritional index available for the labeling of food and biological fluids due to the lack of standardized quantitation methods. The "parent" CUPRAC (CUPric Reducing Antioxidant Capacity) method of antioxidant measurement, introduced by our research group to world literature, is based on the absorbance measurement of Cu(I)neocuproine (Nc) chelate formed as a result of the redox reaction of chain-breaking antioxidants with the CUPRAC reagent, Cu(II)-Nc, where absorbance is recorded at the maximal light absorption wavelength of 450 nm; thus this is an electron-transfer (ET)-based method. From the parent CUPRAC method initially applied to food (apricot, herbal teas, wild edible plants, herby cheese etc.) and biological fluids (as hydrophilic and lipophilic antioxidants together or in separate fractions), a number of "daughter" methods have evolved, such as the simultaneous assay of both lipophilic and hydrophilic antioxidants in acetone-water as methyl-b-cyclodextrin inclusion complexes, determination of ascorbic acid alone in the presence of flavonoids (with preliminary extraction of flavonoids as their La(III)complexes), determination of hydroxyl radical scavenging activity of both water-soluble antioxidants (using benzoate derivatives and salicylate as hydroxylation probes) and of polyphenols using catalase to stop the Fenton reaction so as to prevent redox cycling of antioxidants, measurement of Cu(II)-catalyzed hydrogen peroxide scavenging activity and of xanthine oxidase inhibition activity of polyphenols, TAC measurement of protein thiols in urea buffer, development of a CUPRAC-based antioxidant sensor on a Nafion cation-exchanger membrane, the off-line HPLC-CUPRAC assay and finally the on-line HPLC-CUPRAC assay of antioxidants with post-column detection. The current direction of CUPRAC methodology can be best described as a self-sufficient and integrated train of measurements providing a useful "antioxidant and antiradical assay package". This review attempts to unify and summarize various methodologies of main and modified CUPRAC procedures that can normally be extracted from quite different literature sources.
dc.language.isoeng
dc.subjectZiraat
dc.subjectGıda Mühendisliği
dc.subjectAnalitik Kimya
dc.subjectFizikokimya
dc.subjectSpektroskopi
dc.subjectTemel Bilimler
dc.subjectMühendislik ve Teknoloji
dc.subjectTarım ve Çevre Bilimleri (AGE)
dc.subjectSPEKTROSKOPİ
dc.subjectTarımsal Bilimler
dc.subjectTarım Bilimleri
dc.subjectGIDA BİLİMİ VE TEKNOLOJİSİ
dc.subjectTemel Bilimler (SCI)
dc.subjectKimya
dc.subjectKİMYA, ANALİTİK
dc.titleA comprehensive review of CUPRAC methodology
dc.typeMakale
dc.relation.journalANALYTICAL METHODS
dc.contributor.departmentFatih Sultan Mehmet Vakıf Üniversitesi , ,
dc.identifier.volume3
dc.identifier.issue11
dc.identifier.startpage2439
dc.identifier.endpage2453
dc.contributor.firstauthorID11114


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