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dc.contributor.authorAL ABDULKAREEM, I.
dc.contributor.authorUyar, Fatma Aytül
dc.contributor.authorAL JUMAH, M.
dc.contributor.authorALHAIDAN, Y.
dc.contributor.authorALABDULRAHMAN, A.
dc.contributor.authorHajeer, A. H.
dc.contributor.authorAL BALWI, M. A.
dc.date.accessioned2021-03-05T12:20:38Z
dc.date.available2021-03-05T12:20:38Z
dc.date.issued2013
dc.identifier.citationHajeer A. H. , AL BALWI M. A. , Uyar F. A. , ALHAIDAN Y., ALABDULRAHMAN A., AL ABDULKAREEM I., AL JUMAH M., "HLA-A, -B, -C,-DRB1 and-DQB1 allele and haplotype frequencies in Saudis using next generation sequencing technique", TISSUE ANTIGENS, cilt.82, ss.252-258, 2013
dc.identifier.issn0001-2815
dc.identifier.othervv_1032021
dc.identifier.otherav_acaf43dd-afdb-4c66-bbf0-7da48e416ec3
dc.identifier.urihttp://hdl.handle.net/20.500.12627/115244
dc.identifier.urihttps://doi.org/10.1111/tan.12200
dc.description.abstractNext generation sequencing (NGS) is a promising technique that can reveal the entire gene sequences and to the highest possible resolution without any phase ambiguities. We have used this technique to investigate the frequencies of HLA-A, -B, -C, -DRB1 and -DQB1 in a Saudi cohort of healthy individuals. We used NGS using the 454 genome sequence (GS) FLX System and Conexio assign atf 454 software to human leukocyte antigen (HLA) genotype eight class I and class II loci. A total of 158 healthy Saudi adults were analyzed. The most frequently observed allele for HLA-A was HLA-A*02:01:01:01 (13.6%); for HLA-B, HLA-B*50:01:01 (15.8%); for HLA-C, HLA-C*06:02:01:01 (18.7%); for HLA-DRB1, HLA-DRB1*07:01:01:01 (26.6%); and for HLA-DQB1, HLA-DQB1*02:01:01 (20.3%). The most common four loci haplotypes in the Saudi population were HLA-A*24:02:01:01-B*08:01:01-C*07:02:01:01-DRB1*03:01:01:01 and HLA-A*23:01:01-B*50:01:01-C*06:02:01:01-DRB1*07:01:01:01.. We have used a highly informative technique for HLA typing of a Saudi healthy cohort to establish allele and haplotype frequencies. These results should prove useful for population studies, disease associations and future planning of the unrelated bone marrow donor registry.
dc.language.isoeng
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyokimya
dc.subjectHistoloji-Embriyoloji
dc.subjectCerrahi Tıp Bilimleri
dc.subjectPatoloji
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectTemel Bilimler
dc.subjectBiyoloji ve Biyokimya
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectPATOLOJİ
dc.subjectİmmünoloji
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectHÜCRE BİYOLOJİSİ
dc.titleHLA-A, -B, -C,-DRB1 and-DQB1 allele and haplotype frequencies in Saudis using next generation sequencing technique
dc.typeMakale
dc.relation.journalTISSUE ANTIGENS
dc.contributor.departmentKing Saud Bin Abdulaziz University for Health Sciences , ,
dc.identifier.volume82
dc.identifier.issue4
dc.identifier.startpage252
dc.identifier.endpage258
dc.contributor.firstauthorID43658


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