dc.contributor.author | Marakli, Sevgi | |
dc.contributor.author | Karlik, Elif | |
dc.contributor.author | Guner, Buket Cakmak | |
dc.contributor.author | Gozukirmizi, Nermin | |
dc.date.accessioned | 2021-03-05T11:49:22Z | |
dc.date.available | 2021-03-05T11:49:22Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Guner B. C. , Karlik E., Marakli S., Gozukirmizi N., "Detection of HERV-K6 and HERV-K11 transpositions in the human genome", BIOMEDICAL REPORTS, cilt.9, ss.53-59, 2018 | |
dc.identifier.other | av_aa1f3cfb-bdb0-4be1-bdaf-0e9f901c97a4 | |
dc.identifier.other | vv_1032021 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12627/113602 | |
dc.identifier.uri | https://doi.org/10.3892/br.2018.1096 | |
dc.description.abstract | Mobile genetic elements classed as transposons comprise an estimated 45% of the human genome, and 8% of these elements are human endogenous retroviruses (HERVs). Endogenous retroviruses are retrotransposons, containing 5' and 3' long terminal repeat sequences and encoding envelope, group-specific antigen and DNA polymerase proteins. The aim of the present study was to analyse genome integration polymorphisms of HERV type K member 6 (HERV-K6) and HERV-K11 by using the retrotransposon based molecular marker technique, inter-retrotransposon amplified polymorphism (IRAP). For this purpose, blood samples of 18 healthy individuals within the age range of 10-79 years (10 females and 8 males) were collected, genomic DNAs were isolated and IRAP-polymerase chain reaction (PCR) was performed. IRAP-PCR analyses demonstrated that there were 0-70% polymorphism rates for HERV-K6, and 0-38% polymorphism rates for HERV-K11 among all the samples. Furthermore, the polymorphism rates were 0-70% among females and 11-60% among males for HERV-K6, and 0-38% among females and 0-25% among males for HERV-K11. Age-associated polymorphism was also investigated, but no age-associated polymorphism was observed among the samples. Therefore, HERV-K6 and HERV-K11 polymorphisms may arise on an individual-specific basis. Various previous studies have investigated the associations between the expression of HERVs and cancer or other major diseases. However, few reports have analysed HERV-K movements among individuals. This is the first report to investigate HERV-K6 and HERV-K11 retrotransposon polymorphisms between the genders and different age groups. | |
dc.language.iso | eng | |
dc.subject | TIP, ARAŞTIRMA VE DENEYSEL | |
dc.subject | Klinik Tıp (MED) | |
dc.subject | Tıp | |
dc.subject | Sağlık Bilimleri | |
dc.subject | Dahili Tıp Bilimleri | |
dc.subject | Tıbbi Ekoloji ve Hidroklimatoloji | |
dc.subject | Klinik Tıp | |
dc.title | Detection of HERV-K6 and HERV-K11 transpositions in the human genome | |
dc.type | Makale | |
dc.relation.journal | BIOMEDICAL REPORTS | |
dc.contributor.department | İstanbul Üniversitesi , , | |
dc.identifier.volume | 9 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 53 | |
dc.identifier.endpage | 59 | |
dc.contributor.firstauthorID | 254291 | |