First report on the diagnosis of infectious pancreatic necrosis (IPN) based on reverse transcription polymerase chain reaction (RTPCR) in Turkey

Abstract

Rainbow trout (Oncorhynchus mykiss) farming in Turkey has been widely affected by disease and high mortality of fry since 1990. Several diagnostic investigations have been undertaken and viral particles were identified in electron microscopic studies. In this study, rainbow trout fry that showed the typical signs of IPN were collected from different farms in Turkey and RTPCR performed using two different primer sequences. After the isolation of viral RNA from different fish tissue, amplification product was visualized with silver-stained polyacrylamide gel electrophoresis and 263 bp IPN positive bands were recognised. This RT-PCR assay not only demonstrated the existence of IPN in Turkey but also proved to be a rapid and sensitive technique for the identification of the IPN virus from infected fish tissue.