An HPLC Method for the Determination of Bisoprolol in Human Plasma and its Application to a Pharmacokinetic Study

Abstract

A new high-performance liquid chromatographic method is described for the determination of bisoprolol in human plasma. The proposed method was based on the derivatization of bisoprolol with 4-chloro-7-nitro-2,1,3-benzoxadiazole in borate buffer at pH 9.5 to yield a fluorescent product.