Full automation of Ga-68 labelling of DOTA-peptides including cation exchange prepurification
Tarih
2010Yazar
Petrik, M.
Ocak, M.
Kunkel, F.
Decristoforo, C.
Bergisadi, N.
Antretter, M.
Knopp, R.
Üst veri
Tüm öğe kaydını gösterÖzet
Here we describe a fully automated approach for the synthesis of Ga-68-labelled DOTA-peptides based on pre-concentration and purification of the generator eluate by using a cation exchange-cartridge and its comparison with fully automated direct labelling applying fractionated elution. Pre-concentration of the eluate on a cation exchange cartridge both using a resin-based and a disposable cation-exchange cartridge efficiently removed Ge-68 as well as major metal contaminations with Fe and Zn. This resulted in a high labelling efficiency of DOTA-peptides at high specific activity (SA) with short synthesis times. (C) 2009 Elsevier Ltd. All rights reserved.
Koleksiyonlar
- Makale [92796]