DETERMINATION OF BARLEY HYBRIDS WITH MOLECULAR MARKERS

Abstract

Wild lines and cultivars of barley (Hordeum vulgare, L. cvs. Kaya, Quantum, Tokak, Yercil and Cumhuriyet) and their hybrids were characterized by random amplified polymorphic DNA assay. DNA's isolated from parents and F-1 hybrids were amplified using ten base long primers by a polymerase chain reaction (PCR). Hybrids giving selective banding patterns from both the cultivars and wild parents were taken as real hybrids. This technique is convenient for plant breeders since it is rapid, sensitive and inexpensive.