Cell-free supernatants of lactobacilli inhibit methicilin-resistant staphylococcus aureus, vancomycin-resistant enterococcus and carbapenem-resistant klebsiella strains
Tarih
2021Yazar
Adaleti, Rıza
Gümüş, Defne
Bayırlı Turan, Derya
Küçüker, A.Mine
Nakipoğlu, Yaşar
Kalaycı Yüksek, Fatma
Üst veri
Tüm öğe kaydını gösterÖzet
Aim: Antibiotic resistance is a major health problem. Recently, probiotics are used in the field of alternative/supportive medicine. Thus, in this study, we aimed to evaluate the in vitro inhibitory effects of four different cell-free supernatants (CFSs) of lactobacilli species (L. fermentum, L. plantarum, L. acidophilus and L. rhamnosus) on clinically isolated Methicilin-resistant Staphylococcus aureus (MRSA) strains, Vancomycin-resistant Enterococcus (VRE) strains, and Carbapenem-resistant Klebsiella (CRK) strains.Materials and Methods: Lactobacillus strains were grown in de Man Rogosa Sharpe broth; after filtration, CFSs were diluted to obtain 25, 50, and 100% concentrations. Pathogen bacteria were grown in tryptic soy broth with and without CFSs in a micro-plate. The bacterial growths were measured using spectrophotometric method after four hours of incubation at 37°C. One-way ANOVA followed by Dunnett’s multiple comparisons and Kruskal-Wallis test were used for statistical analyses.Results: All tested CFSs at all concentrations were found to inhibit growth of MRSA, VRE, CRK strains; the results were found statistically significant (p<0.0001). At 50% concentrations, all CFSs were found to be most effective on MRSA growth. The CFSs of L.fermentum, L.acidophilus and L.plantarum were found to be most inhibitory at 50% concentration on VRE growth. 50% diluted CFSs of L.fermentum and L.plantarum were found to be effective on growth of CRK. All results were found statistically significant (p<0.0001).Conclusion: In our study, our results support that CFSs of lactobacilli strains inhibit growth of multi-drug resistant bacteria. Their inhibitory effects were dependent on microorganisms and CFS concentrations.
Bağlantı
http://hdl.handle.net/20.500.12627/180464https://avesis.istanbul.edu.tr/api/publication/d4e536fe-8f82-4de8-8c22-83cc8934bb1c/file
Koleksiyonlar
- Makale [2276]